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Journal of Bacteriology, July 2002, p. 3530-3538, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3530-3538.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Roles for Sigma Factors in Global Circadian Regulation of the Cyanobacterial Genome

Usha Nair,,{dagger} Jayna L. Ditty, Hongtao Min, and Susan S. Golden*

Department of Biology, Texas A&M University, College Station, Texas 77843-3258

Received 14 January 2002/ Accepted 4 April 2002

The circadian clock of the unicellular cyanobacterium Synechococcus elongatus PCC 7942 imposes a global rhythm of transcription on promoters throughout the genome. Inactivation of any of the four known group 2 sigma factor genes (rpoD2, rpoD3, rpoD4, and sigC), singly or pairwise, altered circadian expression from the psbAI promoter, changing amplitude, phase angle, waveform, or period. However, only the rpoD2 mutation and the rpoD3 rpoD4 and rpoD2 rpoD3 double mutations affected expression from the kaiB promoter. A striking differential effect was a 2-h lengthening of the circadian period of expression from the promoter of psbAI, but not of those of kaiB or purF, when sigC was inactivated. The data show that separate timing circuits with different periods can coexist in a cell. Overexpression of rpoD2, rpoD3, rpoD4, or sigC also changed the period or abolished the rhythmicity of PpsbAI expression, consistent with a model in which sigma factors work as a consortium to convey circadian information to downstream genes.

* Corresponding author. Mailing address: Department of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843-3258. Phone: (979) 845-9824. Fax: (979) 862-7659. E-mail: sgolden{at}tamu.edu.

{dagger} Present address: Department of Biology, University of Michigan, Ann Arbor, MI 48109.

Journal of Bacteriology, July 2002, p. 3530-3538, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3530-3538.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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