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Yop Fusions to Tightly Folded Protein Domains and Their Effects on Yersinia enterocolitica Type III Secretion

Vincent T. Lee^, and Olaf Schneewind^*

Department of Microbiology & Immunology, University of California Los Angeles School of Medicine, Los Angeles, California 90095

Received 14 December 2001/ Accepted 2 April 2002

Yersinia enterocolitica organisms secrete Yop proteins via the type III pathway. Translational fusion of yop genes to ubiquitin or dihydrofolate reductase results in hybrid proteins that cannot be secreted. The folding of hybrids prevents their own transport, but it does not hinder the type III secretion of other Yops.

Present address: Department of Microbiology & Molecular Genetics, Harvard Medical School, Boston, MA 02115.

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