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Journal of Bacteriology, July 2002, p. 3753-3755, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3753-3755.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phage {lambda} Red-Mediated Adaptive Mutation

Anthony R. Poteete,1* Hsinju R. Wang,1 and Patricia L. Foster2

Department of Molecular Genetics & Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655,,1 Department of Biology, Indiana University, Bloomington, Indiana 47405-37002

Received 29 January 2002/ Accepted 26 March 2002

Replacement of the recBCD genes of Escherichia coli with the red recombination genes of bacteriophage lambda results in a strain in which adaptive mutation occurs at an elevated frequency. Like RecBCD-dependent adaptive mutation, Red-mediated adaptive mutation is dependent upon recA and ruvABC functions.

* Corresponding author. Mailing address: Dept. of Molecular Genetics & Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655. Phone: (508) 856-3708. Fax: (508) 856-5920. E-mail: anthony.poteete{at}umassmed.edu.

Journal of Bacteriology, July 2002, p. 3753-3755, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3753-3755.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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