This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dinamarca, M. A. Articles by Rojo, F. Search for Related Content PubMed PubMed Citation Articles by Dinamarca, M. A. Articles by Rojo, F.

Inactivation of Cytochrome o Ubiquinol Oxidase Relieves Catabolic Repression of the Pseudomonas putida GPo1 Alkane Degradation Pathway

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

Received 22 March 2002/ Accepted 24 April 2002

Expression of the alkane degradation pathway encoded by the OCT plasmid of Pseudomonas putida GPo1 is regulated by two control systems. One relies on the transcriptional regulator AlkS, which activates expression of the pathway in the presence of alkanes. The other, which is a dominant global regulation control, represses the expression of the pathway genes when a preferred carbon source is present in the growth medium in addition to alkanes. This catabolite repression control occurs through a poorly characterized mechanism that ultimately regulates transcription from the two AlkS-activated promoters of the pathway. To identify the factors involved, a screening method was developed to isolate mutants without this control. Several isolates were obtained, all of which contained mutations that mapped to genes encoding cytochrome o ubiquinol oxidase, the main terminal oxidase of the electron transport chain under highly aerobic conditions. Elimination of this terminal oxidase led to a decrease in the catabolic repression observed both in rich Luria-Bertani medium and in a defined medium containing lactate or succinate as the carbon source. This suggests that catabolic repression could monitor the physiological or metabolic status by using information from the electron transport chain or from the redox state of the cell. Since inactivation of the crc gene also reduces catabolic repression in rich medium (although not that observed in a defined medium), a strain was generated lacking both the Crc function and the cytochrome o terminal oxidase. The two mutations had an additive effect in relieving catabolic repression in rich medium. This suggests that crc and cyo belong to different regulation pathways, both contributing to catabolic repression.

This article has been cited by other articles:

• Galan, B., Manso, I., Kolb, A., Garcia, J. L., Prieto, M. A. (2008). The role of FIS protein in the physiological control of the expression of the Escherichia coli meta-hpa operon. Microbiology 154: 2151-2160 [Abstract] [Full Text]  
• Miyakoshi, M., Shintani, M., Terabayashi, T., Kai, S., Yamane, H., Nojiri, H. (2007). Transcriptome Analysis of Pseudomonas putida KT2440 Harboring the Completely Sequenced IncP-7 Plasmid pCAR1. J. Bacteriol. 189: 6849-6860 [Abstract] [Full Text]  
• del Castillo, T., Ramos, J. L. (2007). Simultaneous Catabolite Repression between Glucose and Toluene Metabolism in Pseudomonas putida Is Channeled through Different Signaling Pathways. J. Bacteriol. 189: 6602-6610 [Abstract] [Full Text]  
• Putrins, M., Tover, A., Tegova, R., Saks, U., Kivisaar, M. (2007). Study of factors which negatively affect expression of the phenol degradation operon pheBA in Pseudomonas putida. Microbiology 153: 1860-1871 [Abstract] [Full Text]  
• Duque, E., Rodriguez-Herva, J.-J., de la Torre, J., Dominguez-Cuevas, P., Munoz-Rojas, J., Ramos, J.-L. (2007). The RpoT Regulon of Pseudomonas putida DOT-T1E and Its Role in Stress Endurance against Solvents. J. Bacteriol. 189: 207-219 [Abstract] [Full Text]  
• Reva, O. N., Weinel, C., Weinel, M., Bohm, K., Stjepandic, D., Hoheisel, J. D., Tummler, B. (2006). Functional Genomics of Stress Response in Pseudomonas putida KT2440. J. Bacteriol. 188: 4079-4092 [Abstract] [Full Text]  
• Doughty, D. M., Sayavedra-Soto, L. A., Arp, D. J., Bottomley, P. J. (2006). Product Repression of Alkane Monooxygenase Expression in Pseudomonas butanovora.. J. Bacteriol. 188: 2586-2592 [Abstract] [Full Text]  
• Munoz-Rojas, J., Bernal, P., Duque, E., Godoy, P., Segura, A., Ramos, J.-L. (2006). Involvement of Cyclopropane Fatty Acids in the Response of Pseudomonas putida KT2440 to Freeze-Drying. Appl. Environ. Microbiol. 72: 472-477 [Abstract] [Full Text]  
• Aranda-Olmedo, I., Ramos, J. L., Marques, S. (2005). Integration of Signals through Crc and PtsN in Catabolite Repression of Pseudomonas putida TOL Plasmid pWW0. Appl. Environ. Microbiol. 71: 4191-4198 [Abstract] [Full Text]  
• Ruiz-Manzano, A., Yuste, L., Rojo, F. (2005). Levels and Activity of the Pseudomonas putida Global Regulatory Protein Crc Vary According to Growth Conditions. J. Bacteriol. 187: 3678-3686 [Abstract] [Full Text]  
• Velazquez, F., di Bartolo, I., de Lorenzo, V. (2004). Genetic Evidence that Catabolites of the Entner-Doudoroff Pathway Signal C Source Repression of the {sigma}54 Pu Promoter of Pseudomonas putida. J. Bacteriol. 186: 8267-8275 [Abstract] [Full Text]  
• Martinez-Perez, O., Moreno-Ruiz, E., Floriano, B., Santero, E. (2004). Regulation of Tetralin Biodegradation and Identification of Genes Essential for Expression of thn Operons. J. Bacteriol. 186: 6101-6109 [Abstract] [Full Text]  
• Dinamarca, M. A., Aranda-Olmedo, I., Puyet, A., Rojo, F. (2003). Expression of the Pseudomonas putida OCT Plasmid Alkane Degradation Pathway Is Modulated by Two Different Global Control Signals: Evidence from Continuous Cultures. J. Bacteriol. 185: 4772-4778 [Abstract] [Full Text]  
• Brzostowicz, P. C., Reams, A. B., Clark, T. J., Neidle, E. L. (2003). Transcriptional Cross-Regulation of the Catechol and Protocatechuate Branches of the {beta}-Ketoadipate Pathway Contributes to Carbon Source-Dependent Expression of the Acinetobacter sp. Strain ADP1 pobA Gene. Appl. Environ. Microbiol. 69: 1598-1606 [Abstract] [Full Text]