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Journal of Bacteriology, July 2002, p. 3931-3940, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3931-3940.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120

Olga A. Koksharova,{dagger} and C. Peter Wolk*

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Received 26 December 2001/ Accepted 24 April 2002

As an approach towards elucidation of the biochemical regulation of the progression of heterocyst differentiation in Anabaena sp. strain PCC 7120, we have identified proteins that bind to a 150-bp sequence upstream from hepC, a gene that plays a role in the synthesis of heterocyst envelope polysaccharide. Such proteins were purified in four steps from extracts of vegetative cells of Anabaena sp. Two of these proteins (Abp1 and Abp2) are encoded by neighboring genes in the Anabaena sp. chromosome. The genes that encode the third (Abp3) and fourth (Abp4) proteins are situated at two other loci in that chromosome. Insertional mutagenesis of abp2 and abp3 blocked expression of hepC and hepA and prevented heterocyst maturation and aerobic fixation of N2.

* Corresponding author. Mailing address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48823. Phone: (517) 353-2049. Fax: (517) 353-9168. E-mail: wolk{at}msu.edu.

{dagger} Permanent address: N. Vavilov Institute of General Genetics, Moscow 119991, Russia.

Journal of Bacteriology, July 2002, p. 3931-3940, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3931-3940.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Lechno-Yossef, S., Fan, Q., Ehira, S., Sato, N., Wolk, C. P. (2006). Mutations in Four Regulatory Genes Have Interrelated Effects on Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 7387-7395 [Abstract] [Full Text]  
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