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Journal of Bacteriology, July 2002, p. 4025-4032, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.4025-4032.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Microarray Analysis of the Mycobacterium tuberculosis Transcriptional Response to the Acidic Conditions Found in Phagosomes

Mark A. Fisher,^1, Bonnie B. Plikaytis,² and Thomas M. Shinnick^1,2*

Program in Microbiology and Molecular Genetics, Emory University, Atlanta, Georgia 30322,¹ Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333²

Received 28 September 2001/ Accepted 22 April 2002

We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.

Present address: Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, Hamilton, MT 59840.

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