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Journal of Bacteriology, July 2002, p. 4025-4032, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.4025-4032.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Microarray Analysis of the Mycobacterium tuberculosis Transcriptional Response to the Acidic Conditions Found in Phagosomes

Mark A. Fisher,1,{dagger} Bonnie B. Plikaytis,2 and Thomas M. Shinnick1,2*

Program in Microbiology and Molecular Genetics, Emory University, Atlanta, Georgia 30322,1 Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332

Received 28 September 2001/ Accepted 22 April 2002

We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.

* Corresponding author. Mailing address: Mailstop G35, Centers for Disease Control and Prevention, 1600 Clifton Rd., N.E., Atlanta, GA 30333. Phone: (404) 639-3601. Fax: (404) 639-1287. E-mail: tms1{at}cdc.gov.

{dagger} Present address: Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, Hamilton, MT 59840.

Journal of Bacteriology, July 2002, p. 4025-4032, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.4025-4032.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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