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Journal of Bacteriology, August 2002, p. 4054-4064, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4054-4064.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Importance of Different tfd Genes for Degradation of Chloroaromatics by Ralstonia eutropha JMP134{dagger}

Iris Plumeier,1 Danilo Pérez-Pantoja,2 Sabina Heim,1 Bernardo González,2 and Dietmar H. Pieper1*

Department of Environmental Biotechnology, GBF-German Research Center for Biotechnology, D-38124 Braunschweig, Germany,1 Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile2

Received 26 December 2001/ Accepted 18 March 2002

The tfdCIDIEIFI, and tfdDIICIIEIIFII gene modules of plasmid pJP4 of Ralstonia eutropha JMP134 encode complete sets of functional enzymes for the transformation of chlorocatechols into 3-oxoadipate, which are all expressed during growth on 2,4-dichlorophenoxyacetate (2,4-D). However, activity of tfdI-encoded enzymes was usually higher than that of tfdII-encoded enzymes, both in the wild-type strain grown on 2,4-D and in 3-chlorobenzoate-grown derivatives harboring only one tfd gene module. The tfdDII-encoded chloromuconate cycloisomerase exhibited special kinetic properties, with high activity against 3-chloromuconate and poor activity against 2-chloromuconate and unsubstituted muconate, thus explaining the different phenotypic behaviors of R. eutropha strains containing different tfd gene modules. The enzyme catalyzes the formation of an equilibrium between 2-chloromuconate and 5-chloro- and 2-chloromuconolactone and very inefficiently catalyzes dehalogenation to form trans-dienelactone as the major product, thus differing from all (chloro)muconate cycloisomerases described thus far.

* Corresponding author. Mailing address: Bereich Mikrobiologie, AG Biodegradation, Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany. Phone: 49/(0)531/6181-467. Fax: 49/(0)531/6181-411. E-mail: dpi{at}gbf.de.

{dagger} For a commentary on this article, see page 4049 in this issue.

Journal of Bacteriology, August 2002, p. 4054-4064, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4054-4064.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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