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Journal of Bacteriology, August 2002, p. 4288-4295, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4288-4295.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability

Ulrike Mäder,* Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, and Michael Hecker

Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, D-17487 Greifswald, Germany

Received 16 January 2002/ Accepted 9 May 2002

A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional protein gel electrophoresis and DNA macroarray technology were combined to analyze cells exponentially grown in minimal medium with and without 0.2% Casamino Acids (CAA). This approach revealed about 120 genes predominantly involved in amino acid biosynthesis, sporulation, and competence, which were downregulated in CAA-containing medium. Determination of sporulation frequencies confirmed the physiological relevance of the expression data.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, F.-L.-Jahn-Str. 15, D-17487 Greifswald, Germany. Phone: 49-3834-864164. Fax: 49-3834-864172. E-mail: ulrike.maeder{at}uni-greifswald.de.

Journal of Bacteriology, August 2002, p. 4288-4295, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4288-4295.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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