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Journal of Bacteriology, August 2002, p. 4301-4303, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4301-4303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Glycine Betaine Transmethylase Mutant of Pseudomonas aeruginosa

Ana L. Serra,1 Javier F. Mariscotti,1 José L. Barra,2 Gloria I. Lucchesi,1 Carlos E. Domenech,1 and Angela T. Lisa1*

Departmento de Biologíia Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5.800 Río Cuarto,1 Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5000 Córdoba, Argentina2

Received 21 December 2001/ Accepted 30 April 2002

The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.

* Corresponding author. Mailing address: Departmento de Biologíia Molecular, Facultad de Ciencas Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5.800 Río Cuarto, Argentina. Phone and fax: 54 358 4676232. E-mail: tlisa{at}exa.unrc.edu.ar.

Journal of Bacteriology, August 2002, p. 4301-4303, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4301-4303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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