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Journal of Bacteriology, August 2002, p. 4304-4307, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4304-4307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Aquaporin Z of Escherichia coli: Reassessment of Its Regulation and Physiological Role

Eric Soupene, Natalie King, Haidy Lee,,{dagger} and Sydney Kustu*

Department of Plant and Microbial Biology, University of California, Berkeley, California 94720

Received 25 March 2002/ Accepted 29 April 2002

Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- or downshifts in osmolality. Disruption of aqpZ had no detectable adverse effects.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, University of California, 111 Koshland Hall no. 3102, Berkeley, CA 94720-3102. Phone: (510) 643-9308. Fax: (510) 642-4995. E-mail: kustu{at}nature.berkeley.edu.

{dagger} Present address: University of California, Irvine, College of Medicine, Irvine, CA 92697.

Journal of Bacteriology, August 2002, p. 4304-4307, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4304-4307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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