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Journal of Bacteriology, August 2002, p. 4308-4312, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4308-4312.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The MexR Repressor of the mexAB-oprM Multidrug Efflux Operon in Pseudomonas aeruginosa: Characterization of Mutations Compromising Activity

Lateef Adewoye, Ainsley Sutherland, Ramakrishnan Srikumar, and Keith Poole*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Received 15 November 2001/ Accepted 10 May 2002

Mutations in mexR yield a multidrug resistance phenotype in nalB mutants of Pseudomonas aeruginosa as a result of derepression of the mexAB-oprM multidrug efflux operon. MexR produced by several nalB strains carried single amino acid changes that compromised MexR stability or its ability to dimerize. Changes at residues L95 and R21, however, produced a stable MexR protein capable of dimerization and, thus, likely compromised DNA binding.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6. Phone: (613) 533-6677. Fax: (613) 533-6796. E-mail: poolek{at}post.queensu.ca.

Journal of Bacteriology, August 2002, p. 4308-4312, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4308-4312.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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