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Journal of Bacteriology, August 2002, p. 4617-4619, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4617-4619.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

One Repeat of the Cell Wall Binding Domain Is Sufficient for Anchoring the Lactobacillus acidophilus Surface Layer Protein

Egbert Smit and Peter H. Pouwels*

TNO Nutrition and Food Research Institute, Department of Applied Microbiology and Gene Technology, 3700 AJ Zeist, The Netherlands

Received 23 April 2002/ Accepted 17 May 2002

The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid.

* Corresponding author. Mailing address: TNO Prevention and Health, Zernikedreef 9, P.O. Box 2215, 2301 CE Leiden, The Netherlands. Phone: 31 71 518 1818. Fax: 31 71 518 1901. E-mail: p.pouwels{at}pg.tno.nl.

Journal of Bacteriology, August 2002, p. 4617-4619, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4617-4619.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Neuhaus, F. C., Baddiley, J. (2003). A Continuum of Anionic Charge: Structures and Functions of D-Alanyl-Teichoic Acids in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 686-723 [Abstract] [Full Text]  


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