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Journal of Bacteriology, August 2002, p. 4620-4625, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4620-4625.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutational Analysis of the TolA C-Terminal Domain of Escherichia coli and Genetic Evidence for an Interaction between TolA and TolB

Jean François Dubuisson, Anne Vianney, and Jean Claude Lazzaroni*

Unité de Microbiologie et Génétique, UMR5122 (CNRS-INSA-Université Lyon1), F-69622 Villeurbanne Cedex, France

Received 7 March 2002/ Accepted 11 May 2002

The Tol proteins are involved in the outer membrane stability of gram-negative bacteria. The C-terminal domain of TolA was mutagenized to identify residues important for its functions. The isolation of suppressor mutants of tolA mutations in the tolB gene confirmed an interaction between TolAIII and the N-terminal domain of TolB.


* Corresponding author. Mailing address: Unité de Microbiologie et Génétique, UMR5122 CNRS, University of Lyon, bÂt André Lwoff, 10, rue Dubois, F-69622 Villeurbanne Cedex, France. Phone: (33) 472 43 13 67. Fax: (33) 472 43 26 86. E-mail: lazzaroni{at}biomserv.univ-lyon1.fr.


Journal of Bacteriology, August 2002, p. 4620-4625, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4620-4625.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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