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Journal of Bacteriology, August 2002, p. 4640-4643, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4640-4643.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The NorR Protein of Escherichia coli Activates Expression of the Flavorubredoxin Gene norV in Response to Reactive Nitrogen Species

Matthew I. Hutchings,^, Neeraj Mandhana, and Stephen Spiro^*

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom

Received 28 March 2002/ Accepted 9 May 2002

The Escherichia coli norVW genes encode a flavorubredoxin and NADH:(flavo)rubredoxin reductase, respectively, which are involved in nitric oxide detoxification under anaerobic growth conditions. Here it is shown that the norVW genes also have a role in protection against reactive nitrogen intermediates generated from nitroprusside. Transcription from the norV promoter is activated by the presence of nitroprusside in the growth medium; activation requires the product of a divergently transcribed regulatory gene, norR.

Present address: John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom.

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