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Journal of Bacteriology, September 2002, p. 4690-4698, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4690-4698.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genomic Variability of O Islands Encoding Tellurite Resistance in Enterohemorrhagic Escherichia coli O157:H7 Isolates

Diane E. Taylor,1* Michelle Rooker,1 Monika Keelan,1 Lai-King Ng,2 Irene Martin,2 Nicole T. Perna,3,{dagger} N. T. Valerie Burland,3 and Fredrick R. Blattner3

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7 ,1 National Microbiology Laboratory, Winnipeg, Manitoba R3E 3R2, Canada,2 Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 537063

Received 19 April 2002/ Accepted 4 June 2002

Strains of Escherichia coli causing enterohemorrhagic colitis belonging to the O157:H7 lineage are reported to be highly related. Fifteen strains of E. coli O157:H7 and 1 strain of E. coli O46:H- (nonflagellated) were examined for the presence of potassium tellurite resistance (Ter). Ter genes comprising terABCDEF were shown previously to be part of a pathogenicity island also containing integrase, phage, and urease genes. PCR analysis, both conventional and light cycler based, demonstrated that about one-half of the Ter E. coli O157:H7 strains (6 of 15), including the Sakai strain, which has been sequenced, carried a single copy of the Ter genes. Five of the strains, including EDL933, which has also been sequenced, contained two copies. Three other O157:H7 strains and the O46:H- strain did not contain the Ter genes. In strains containing two copies, the Ter genes were associated with the serW and serX tRNA genes. Five O157:H7 strains resembled the O157 Sakai strain whose sequence contained one copy, close to serX, whereas in one isolate the single copy was associated with serW. There was no correlation between Ter and the ability to produce Shiga toxin ST1 or ST2. The Ter MIC for most strains, containing either one or two copies, was 1,024 µg/ml, although for a few the MIC was intermediate, 64 to 128 µg/ml, which could be increased to 512 µg/ml by pregrowth of strains in subinhibitory concentrations of potassium tellurite. Reverse transcriptase PCR analysis confirmed that in most strains Ter was constitutive but that in the rest it was inducible and involved induction of terB and terC genes. Only the terB, -C, -D, and -E genes are required for Ter. The considerable degree of homology between the ter genes on IncH12 plasmid R478, which originated in Serratia marcescens, and pTE53, from an E. coli clinical isolate, suggests that the pathogenicity island was acquired from a plasmid. This work demonstrates diversity among E. coli O157:H7 isolates, at least as far as the presence of Ter genes is concerned.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 1-28 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-4777. Fax: (780) 492-7521. E-mail: Diane.Taylor{at}ualberta.ca.

{dagger} Present address: Animal Health and Biological Sciences, University of Wisconsin, Madison, Wis.

Journal of Bacteriology, September 2002, p. 4690-4698, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4690-4698.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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