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Journal of Bacteriology, September 2002, p. 4875-4880, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4875-4880.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

SipY Is the Streptomyces lividans Type I Signal Peptidase Exerting a Major Effect on Protein Secretion

Arantxa Palacín,1 Víctor Parro,1,{dagger} Nick Geukens,2 Jozef Anné,2 and Rafael P. Mellado1*

Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain ,1 Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium2

Received 20 December 2001/ Accepted 10 June 2002

Most bacteria contain one type I signal peptidase (SPase) for cleavage of signal peptides from secreted proteins. The developmental complex bacterium Streptomyces lividans has the ability to produce and secrete a significant amount of proteins and has four different type I signal peptidases genes (sipW, sipX, sipY, and sipZ) unusually clustered in its chromosome. Functional analysis of the four SPases was carried out by phenotypical and molecular characterization of the different individual sip mutants. None of the sip genes seemed to be essential for bacterial growth. Analysis of total extracellular proteins indicated that SipY is likely to be the major S. lividans SPase, since the sipY mutant strain is highly deficient in overall protein secretion and extracellular protease production, showing a delayed sporulation phenotype when cultured in solid medium.

* Corresponding author. Mailing address: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain. Phone: 34-915854547. Fax: 34-915854506. E-mail: rpmellado{at}cnb.uam.es.

{dagger} Present address: Centro de Astrobiología, INTA-CSIC, 28850 Torrejón de Ardoz, Madrid, Spain.

Journal of Bacteriology, September 2002, p. 4875-4880, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4875-4880.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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