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Journal of Bacteriology, September 2002, p. 4952-4961, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.4952-4961.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases

S. H. Willis,1 K. M. Kazmierczak,1,{dagger} R. H. Carter,2 and L. B. Rothman-Denes1*

Departments of Molecular Genetics and Cell Biology,1 Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 606372

Received 22 April 2002/ Accepted 14 June 2002

Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.

* Corresponding author. Mailing address: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637. Phone: (773) 702-1083. Fax: (773) 702-3172. E-mail: lbrd{at}midway.uchicago.edu.

{dagger} Present address: Infectious Diseases Research, Lilly Research Laboratories, Indianapolis, IN 46285.

Journal of Bacteriology, September 2002, p. 4952-4961, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.4952-4961.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Carter, R. H., Demidenko, A. A., Hattingh-Willis, S., Rothman-Denes, L. B. (2003). Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein. Genes Dev. 17: 2334-2345 [Abstract] [Full Text]  


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