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Journal of Bacteriology, September 2002, p. 4962-4970, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.4962-4970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Proteome Analysis of Brucella melitensis Vaccine Strain Rev 1 and a Virulent Strain, 16M

Michel Eschenbrenner,1 Mary Ann Wagner,1 Troy A. Horn,1 Jo Ann Kraycer,1 Cesar V. Mujer,1 Sue Hagius,2 Philip Elzer,2 and Vito G. DelVecchio1*

Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510,1 Department of Veterinary Science, Louisiana State University AgCenter, Baton Rouge, Louisiana 708032

Received 14 February 2002/ Accepted 13 June 2002

The genus Brucella consists of bacterial pathogens that cause brucellosis, a major zoonotic disease characterized by undulant fever and neurological disorders in humans. Among the different Brucella species, Brucella melitensis is considered the most virulent. Despite successful use in animals, the vaccine strains remain infectious for humans. To understand the mechanism of virulence in B. melitensis, the proteome of vaccine strain Rev 1 was analyzed by two-dimensional gel electrophoresis and compared to that of virulent strain 16M. The two strains were grown under identical laboratory conditions. Computer-assisted analysis of the two B. melitensis proteomes revealed proteins expressed in either 16M or Rev 1, as well as up- or down-regulation of proteins specific for each of these strains. These proteins were identified by peptide mass fingerprinting. It was found that certain metabolic pathways may be deregulated in Rev 1. Expression of an immunogenic 31-kDa outer membrane protein, proteins utilized for iron acquisition, and those that play a role in sugar binding, lipid degradation, and amino acid binding was altered in Rev 1.

* Corresponding author. Mailing address: Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, PA 18510-4625. Phone: (570) 941-6353. Fax: (570) 941-6229. E-mail: vimbm{at}aol.com.

Journal of Bacteriology, September 2002, p. 4962-4970, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.4962-4970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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