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Journal of Bacteriology, September 2002, p. 5130-5140, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5130-5140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli

Kazushi Suzuki,1 Xin Wang,1 Thomas Weilbacher,1 Anna-Karin Pernestig,2 Öjar Melefors,2 Dimitris Georgellis,3 Paul Babitzke,4 and Tony Romeo1*

Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322,1 Microbiology and Tumorbiology Center, Karolinska Institutet, SE-171 77 Stockholm, Sweden,2 Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico,3 Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 168024

Received 27 March 2002/ Accepted 14 June 2002

The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globular ribonucleoprotein complex. CsrA indirectly activates csrB transcription, in an apparent autoregulatory mechanism. In the present study, we elucidate the intermediate regulatory circuitry of this system. Mutations affecting the BarA/UvrY two-component signal transduction system decreased csrB transcription but did not affect csrA'-'lacZ expression. The uvrY defect was severalfold more severe than that of barA. Both csrA and uvrY were required for optimal barA expression. The latter observation suggests an autoregulatory loop for UvrY. Ectopic expression of uvrY suppressed the csrB-lacZ expression defects caused by uvrY, csrA, or barA mutations; csrA suppressed csrA or barA defects; and barA complemented only the barA mutation. Purified UvrY protein stimulated csrB-lacZ expression approximately sixfold in S-30 transcription-translation reactions, revealing a direct effect of UvrY on csrB transcription. Disruption of sdiA, which encodes a LuxR homologue, decreased the expression of uvrY'-'lacZ and csrB-lacZ fusions but did not affect csrA'-'lacZ. The BarA/UvrY system activated biofilm formation. Ectopic expression of uvrY stimulated biofilm formation by a csrB-null mutant, indicative of a CsrB-independent role for UvrY in biofilm development. Collectively, these results demonstrate that uvrY resides downstream from csrA in a signaling pathway for csrB and that CsrA stimulates UvrY-dependent activation of csrB expression by BarA-dependent and -independent mechanisms.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, 3133 Rollins Research Center, 1510 Clifton Rd. N.E., Atlanta, GA 30322. Phone: (404) 727-5950. Fax: (404) 727-8250. E-mail: romeo{at}microbio.emory.edu.

Journal of Bacteriology, September 2002, p. 5130-5140, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5130-5140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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