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Journal of Bacteriology, September 2002, p. 5174-5178, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5174-5178.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Insufficient Expression of the ilv-leu Operon Encoding Enzymes of Branched-Chain Amino Acid Biosynthesis Limits Growth of a Bacillus subtilis ccpA Mutant

Holger Ludwig, Christoph Meinken, Anastasija Matin, and Jörg Stülke*

Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany

Received 17 April 2002/ Accepted 14 June 2002

Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-chain amino acids for optimal growth. The control of expression of the ilv-leu operon by CcpA provides a novel regulatory link between carbon and amino acid metabolism.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany. Phone: (49) 9131 8528818. Fax: (49) 9131 8528082. E-mail: jstuelke{at}biologie.uni-erlangen.de.

Journal of Bacteriology, September 2002, p. 5174-5178, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5174-5178.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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