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Journal of Bacteriology, September 2002, p. 5187-5193, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5187-5193.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Amplification of the Tetracycline Resistance Determinant of pAM1 in Enterococcus faecalis Requires a Site-Specific Recombination Event Involving Relaxase

M. Victoria Francia¹ and Don B. Clewell^1,2*

Department of Biologic and Materials Sciences, School of Dentistry,¹ Department of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 48109²

Received 19 April 2002/ Accepted 18 June 2002

The small multicopy plasmid pAM1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAM1.

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* Corresponding author. Mailing address: Department of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109-1078. Phone: (734) 763-0117. Fax: (734) 763-9905. E-mail: dclewell{at}umich.edu.

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Journal of Bacteriology, September 2002, p. 5187-5193, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5187-5193.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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