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Amplification of the Tetracycline Resistance Determinant of pAM1 in Enterococcus faecalis Requires a Site-Specific Recombination Event Involving Relaxase

Department of Biologic and Materials Sciences, School of Dentistry,¹ Department of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 48109²

Received 19 April 2002/ Accepted 18 June 2002

The small multicopy plasmid pAM1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAM1.

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