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Journal of Bacteriology, October 2002, p. 5234-5239, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5234-5239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of GtgE, a Novel Virulence Factor Encoded on the Gifsy-2 Bacteriophage of Salmonella enterica Serovar Typhimurium

Theresa D. Ho,1,{dagger} Nara Figueroa-Bossi,2 Minhua Wang,1 Sergio Uzzau,2,{ddagger} Lionello Bossi,2 and James M. Slauch1,3*

Department of Microbiology,1 College of Medicine, University of Illinois, Urbana, Illinois 61801,3 Centre de Genetique, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France2

Received 23 April 2002/ Accepted 2 July 2002

The Gifsy-2 temperate bacteriophage of Salmonella enterica serovar Typhimurium contributes significantly to the pathogenicity of strains that carry it as a prophage. Previous studies have shown that Gifsy-2 encodes SodCI, a periplasmic Cu/Zn superoxide dismutase, and at least one additional virulence factor. Gifsy-2 encodes a Salmonella pathogenicity island 2 type III secreted effector protein. Sequence analysis of the Gifsy-2 genome also identifies several open reading frames with homology to those of known virulence genes. However, we found that null mutations in these genes did not individually have a significant effect on the ability of S. enterica serovar Typhimurium to establish a systemic infection in mice. Using deletion analysis, we have identified a gene, gtgE, which is necessary for the full virulence of S. enterica serovar Typhimurium Gifsy-2 lysogens. Together, GtgE and SodCI account for the contribution of Gifsy-2 to S. enterica serovar Typhimurium virulence in the murine model.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, MC-110, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1956. Fax: (217) 244-6697. E-mail: j-slauch{at}uiuc.edu.

{dagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

{ddagger} Present address: Department of Biomedical Sciences University of Sassari, 07100 Sassari, Italy.

Journal of Bacteriology, October 2002, p. 5234-5239, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5234-5239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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