This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Atkinson, M. R.
Right arrow Articles by Ninfa, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atkinson, M. R.
Right arrow Articles by Ninfa, A. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5358-5363, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5358-5363.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Activation of the glnA, glnK, and nac Promoters as Escherichia coli Undergoes the Transition from Nitrogen Excess Growth to Nitrogen Starvation

Mariette R. Atkinson,1 Timothy A. Blauwkamp,1 Vladamir Bondarenko,2 Vasily Studitsky,2 and Alexander J. Ninfa1*

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 ,1 Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 482012

Received 28 February 2002/ Accepted 24 June 2002

The nitrogen-regulated genes and operons of the Ntr regulon of Escherichia coli are activated by the enhancer-binding transcriptional activator NRI~P (NtrC~P). Here, we examined the activation of the glnA, glnK, and nac promoters as cells undergo the transition from growth on ammonia to nitrogen starvation and examined the amplification of NRI during this transition. The results indicate that the concentration of NRI is increased as cells become starved for ammonia, concurrent with the activation of Ntr genes that have less- efficient enhancers than does glnA. A diauxic growth pattern was obtained when E. coli was grown on a low concentration of ammonia in combination with arginine as a nitrogen source, consistent with the hypothesis that Ntr genes other than glnA become activated only upon amplification of the NRI concentration.

* Corresponding author. Mailing address: Department of Biological Chemistry, University of Michigan Medical School, 1301 E. Catherine, Ann Arbor, MI 48109-0606. Phone: (734) 763-8065. Fax: (734) 763-4581. E-mail: aninfa{at}umich.edu.

Journal of Bacteriology, October 2002, p. 5358-5363, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5358-5363.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hervas, A. B., Canosa, I., Little, R., Dixon, R., Santero, E. (2009). NtrC-Dependent Regulatory Network for Nitrogen Assimilation in Pseudomonas putida. J. Bacteriol. 191: 6123-6135 [Abstract] [Full Text]  
  • Fisher, S. H., Wray, L. V. Jr. (2009). Novel trans-Acting Bacillus subtilis glnA Mutations That Derepress glnRA Expression. J. Bacteriol. 191: 2485-2492 [Abstract] [Full Text]  
  • Mao, X.-J., Huo, Y.-X., Buck, M., Kolb, A., Wang, Y.-P. (2007). Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in Escherichia coli. Nucleic Acids Res 35: 1432-1440 [Abstract] [Full Text]  
  • Tondervik, A., Torgersen, H. R., Botnmark, H. K., Strom, A. R. (2006). Transposon Mutations in the 5' End of glnD, the Gene for a Nitrogen Regulatory Sensor, That Suppress the Osmosensitive Phenotype Caused by otsBA Lesions in Escherichia coli.. J. Bacteriol. 188: 4218-4226 [Abstract] [Full Text]  
  • Kurata, H., Masaki, K., Sumida, Y., Iwasaki, R. (2005). CADLIVE dynamic simulator: Direct link of biochemical networks to dynamic models. Genome Res 15: 590-600 [Abstract] [Full Text]  
  • Pioszak, A. A., Ninfa, A. J. (2004). Mutations Altering the N-Terminal Receiver Domain of NRI (NtrC) That Prevent Dephosphorylation by the NRII-PII Complex in Escherichia coli. J. Bacteriol. 186: 5730-5740 [Abstract] [Full Text]  
  • Martinez-Argudo, I., Little, R., Shearer, N., Johnson, P., Dixon, R. (2004). The NifL-NifA System: a Multidomain Transcriptional Regulatory Complex That Integrates Environmental Signals. J. Bacteriol. 186: 601-610 [Full Text]  
  • Harrod, A. C., Yang, X., Junker, M., Reitzer, L. (2004). Evidence for a Second Interaction between the Regulatory Amino-terminal and Central Output Domains of the Response Regulator NtrC (Nitrogen Regulator I) in Escherichia coli. J. Biol. Chem. 279: 2350-2359 [Abstract] [Full Text]  
  • Maheswaran, M., Forchhammer, K. (2003). Carbon-source-dependent nitrogen regulation in Escherichia coli is mediated through glutamine-dependent GlnB signalling. Microbiology 149: 2163-2172 [Abstract] [Full Text]  
  • Pioszak, A. A., Ninfa, A. J. (2003). Genetic and Biochemical Analysis of Phosphatase Activity of Escherichia coli NRII (NtrB) and Its Regulation by the PII Signal Transduction Protein. J. Bacteriol. 185: 1299-1315 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»