This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Unniraman, S.
Right arrow Articles by Nagaraja, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unniraman, S.
Right arrow Articles by Nagaraja, V.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5449-5456, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5449-5456.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Gyrase Genes in Mycobacterium tuberculosis: a Single Operon Driven by Multiple Promoters

Shyam Unniraman,1,{dagger} Monalisa Chatterji,1 and Valakunja Nagaraja1,2*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012,1 Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India2

Received 15 April 2002/ Accepted 10 July 2002

The two genes encoding DNA gyrase in Mycobacterium tuberculosis are present next to each other in the genome, with gyrB upstream of gyrA. We show that the primary transcript is dicistronic. However, in addition to the principal promoter, there are multiple weaker promoters that appear to fine-tune transcription. With these and other mycobacterial promoters, we propose consensus promoter sequences for two distinct sigma factors. In addition to this, the gyr genes in M. tuberculosis, as in other species, are subject to autoregulation, albeit with slower kinetics, probably reflecting the slower metabolism of the organism.

* Corresponding author. Mailing address: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India. Phone: 91 80 360 0668. Fax: 91 80 360 2697. E-mail: vraj{at}mcbl.iisc.ernet.in.

{dagger} Present address: HHMI/Section of Immunobiology, Yale University Medical School, New Haven, Conn.

Journal of Bacteriology, October 2002, p. 5449-5456, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5449-5456.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chauhan, S., Tyagi, J. S. (2009). Powerful Induction of Divergent tgs1-Rv3131 Genes in Mycobacterium tuberculosis Is Mediated by DevR Interaction with a High-Affinity Site and an Adjacent Cryptic Low-Affinity Site. J. Bacteriol. 191: 6075-6081 [Abstract] [Full Text]  
  • Guillot, C., Moran, C. P. Jr. (2007). Essential Internal Promoter in the spoIIIA Locus of Bacillus subtilis. J. Bacteriol. 189: 7181-7189 [Abstract] [Full Text]  
  • Roback, P., Beard, J., Baumann, D., Gille, C., Henry, K., Krohn, S., Wiste, H., Voskuil, M.I., Rainville, C., Rutherford, R. (2007). A predicted operon map for Mycobacterium tuberculosis. Nucleic Acids Res 35: 5085-5095 [Abstract] [Full Text]  
  • Buroni, S., Manina, G., Guglierame, P., Pasca, M. R., Riccardi, G., De Rossi, E. (2006). LfrR Is a Repressor That Regulates Expression of the Efflux Pump LfrA in Mycobacterium smegmatis. Antimicrob. Agents Chemother. 50: 4044-4052 [Abstract] [Full Text]  
  • Oram, D. M., Jacobson, A. D., Holmes, R. K. (2006). Transcription of the Contiguous sigB, dtxR, and galE Genes in Corynebacterium diphtheriae: Evidence for Multiple Transcripts and Regulation by Environmental Factors.. J. Bacteriol. 188: 2959-2973 [Abstract] [Full Text]  
  • Manganelli, R., Proveddi, R., Rodrigue, S., Beaucher, J., Gaudreau, L., Smith, I. (2004). {sigma} Factors and Global Gene Regulation in Mycobacterium tuberculosis. J. Bacteriol. 186: 895-902 [Full Text]  
  • Recchi, C., Sclavi, B., Rauzier, J., Gicquel, B., Reyrat, J.-M. (2003). Mycobacterium tuberculosis Rv1395 Is a Class III Transcriptional Regulator of the AraC Family Involved in Cytochrome P450 Regulation. J. Biol. Chem. 278: 33763-33773 [Abstract] [Full Text]  
  • Lamichhane, G., Zignol, M., Blades, N. J., Geiman, D. E., Dougherty, A., Grosset, J., Broman, K. W., Bishai, W. R. (2003). A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: Application to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 100: 7213-7218 [Abstract] [Full Text]  
  • Unniraman, S., Chatterji, M., Nagaraja, V. (2002). A hairpin near the 5' end stabilises the DNA gyrase mRNA in Mycobacterium smegmatis. Nucleic Acids Res 30: 5376-5381 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»