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Journal of Bacteriology, October 2002, p. 5449-5456, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5449-5456.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Gyrase Genes in Mycobacterium tuberculosis: a Single Operon Driven by Multiple Promoters

Shyam Unniraman,^1, Monalisa Chatterji,¹ and Valakunja Nagaraja^1,2*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012,¹ Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India²

Received 15 April 2002/ Accepted 10 July 2002

The two genes encoding DNA gyrase in Mycobacterium tuberculosis are present next to each other in the genome, with gyrB upstream of gyrA. We show that the primary transcript is dicistronic. However, in addition to the principal promoter, there are multiple weaker promoters that appear to fine-tune transcription. With these and other mycobacterial promoters, we propose consensus promoter sequences for two distinct sigma factors. In addition to this, the gyr genes in M. tuberculosis, as in other species, are subject to autoregulation, albeit with slower kinetics, probably reflecting the slower metabolism of the organism.

Present address: HHMI/Section of Immunobiology, Yale University Medical School, New Haven, Conn.

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