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Journal of Bacteriology, October 2002, p. 5491-5494, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5491-5494.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Amino Acid Substitutions in KdpA, the K+-Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli

Martin van der Laan,{dagger} Michael Gaßel,{ddagger} and Karlheinz Altendorf*

Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany

Received 19 February 2002/ Accepted 9 July 2002

When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA, which is responsible for K+ binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity.

* Corresponding author. Mailing address: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany. Phone: 49-541-969-2864. Fax: 49-541-969-12891. E-mail: Altendorf{at}biologie.uni-osnabrueck.de.

{dagger} Present address: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands.

{ddagger} Present address: Department of Pathochemistry, German Cancer Research Center, DKFZ, D-69120 Heidelberg, Germany.

Journal of Bacteriology, October 2002, p. 5491-5494, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5491-5494.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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