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Journal of Bacteriology, January 2002, p. 564-571, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.564-571.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Postexponential Regulation of sin Operon Expression in Bacillus subtilis

Department of Molecular and Cellular Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720,¹ Department of Microbiology, Public Health Research Institute, New York, New York 10016,² University of Ljubljana, 6100 Ljubljana, Slovenia,³ Department of Oral and Craniofacial Biological Sciences, University of Maryland, Baltimore, Maryland 21201⁴

Received 12 February 2001/ Accepted 16 October 2001

The expression of many gene products required during the early stages of Bacillus subtilis sporulation is regulated by sinIR operon proteins. Transcription of sinIR from the P1 promoter is induced at the end of exponential growth. In vivo transcription studies suggest that P1 induction is repressed by the transition-state regulatory protein Hpr and is induced by the phosphorylated form of Spo0A. In vitro DNase I footprinting studies confirmed that Hpr, AbrB, and Spo0A are trans-acting transcriptional factors that bind to the P1 promoter region of sinIR. We have also determined that the P1 promoter is transcribed in vitro by the major vegetative sigma factor, ^A, form of RNA polymerase.

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