This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shafikhani, S. H.
Right arrow Articles by Leighton, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shafikhani, S. H.
Right arrow Articles by Leighton, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2002, p. 564-571, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.564-571.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Postexponential Regulation of sin Operon Expression in Bacillus subtilis

Sasha H. Shafikhani,1 Ines Mandic-Mulec,2,3 Mark A. Strauch,4 Issar Smith,2 and Terrance Leighton1*

Department of Molecular and Cellular Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720,1 Department of Microbiology, Public Health Research Institute, New York, New York 10016,2 University of Ljubljana, 6100 Ljubljana, Slovenia,3 Department of Oral and Craniofacial Biological Sciences, University of Maryland, Baltimore, Maryland 212014

Received 12 February 2001/ Accepted 16 October 2001

The expression of many gene products required during the early stages of Bacillus subtilis sporulation is regulated by sinIR operon proteins. Transcription of sinIR from the P1 promoter is induced at the end of exponential growth. In vivo transcription studies suggest that P1 induction is repressed by the transition-state regulatory protein Hpr and is induced by the phosphorylated form of Spo0A. In vitro DNase I footprinting studies confirmed that Hpr, AbrB, and Spo0A are trans-acting transcriptional factors that bind to the P1 promoter region of sinIR. We have also determined that the P1 promoter is transcribed in vitro by the major vegetative sigma factor, {varsigma}A, form of RNA polymerase.

* Corresponding author. Mailing address: Division of Biochemistry and Molecular Biology, 401 Barker Hall, University of California, Berkeley, CA 94720. Phone: (510) 642-1620. Fax: (510) 643-5035. E-mail: leighton{at}bacillus.berkeley.edu.

Journal of Bacteriology, January 2002, p. 564-571, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.564-571.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kodgire, P., Rao, K. K. (2009). hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC. Microbiology 155: 142-149 [Abstract] [Full Text]  
  • Vlamakis, H., Aguilar, C., Losick, R., Kolter, R. (2008). Control of cell fate by the formation of an architecturally complex bacterial community. Genes Dev. 22: 945-953 [Abstract] [Full Text]  
  • Kodgire, P., Dixit, M., Rao, K. K. (2006). ScoC and SinR Negatively Regulate epr by Corepression in Bacillus subtilis.. J. Bacteriol. 188: 6425-6428 [Abstract] [Full Text]  
  • Veening, J.-W., Kuipers, O. P., Brul, S., Hellingwerf, K. J., Kort, R. (2006). Effects of Phosphorelay Perturbations on Architecture, Sporulation, and Spore Resistance in Biofilms of Bacillus subtilis.. J. Bacteriol. 188: 3099-3109 [Abstract] [Full Text]  
  • Gottig, N., Pedrido, M. E., Mendez, M., Lombardia, E., Rovetto, A., Philippe, V., Orsaria, L., Grau, R. (2005). The Bacillus subtilis SinR and RapA Developmental Regulators Are Responsible for Inhibition of Spore Development by Alcohol. J. Bacteriol. 187: 2662-2672 [Abstract] [Full Text]  
  • Voigt, C. A., Wolf, D. M., Arkin, A. P. (2005). The Bacillus subtilis sin Operon: An Evolvable Network Motif. Genetics 169: 1187-1202 [Abstract] [Full Text]  
  • Fujita, M., Gonzalez-Pastor, J. E., Losick, R. (2005). High- and Low-Threshold Genes in the Spo0A Regulon of Bacillus subtilis. J. Bacteriol. 187: 1357-1368 [Abstract] [Full Text]  
  • Ogura, M., Matsuzawa, A., Yoshikawa, H., Tanaka, T. (2004). Bacillus subtilis SalA (YbaL) Negatively Regulates Expression of scoC, Which Encodes the Repressor for the Alkaline Exoprotease Gene, aprE. J. Bacteriol. 186: 3056-3064 [Abstract] [Full Text]  
  • Liu, J., Tan, K., Stormo, G. D. (2003). Computational identification of the Spo0A-phosphate regulon that is essential for the cellular differentiation and development in Gram-positive spore-forming bacteria. Nucleic Acids Res 31: 6891-6903 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»