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Journal of Bacteriology, January 2002, p. 600-604, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.600-604.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

{alpha}-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome

Sadanari Jindou,1 Shuichi Karita,1 Emi Fujino,1 Tsuchiyoshi Fujino,2 Hidenori Hayashi,1 Tetsuya Kimura,1 Kazuo Sakka,1* and Kunio Ohmiya1

Faculty of Bioresources, Mie University, Tsu 514-8507, Japan,1 Nagoya Seiraku Co. Ltd., Nagoya 468-8588, Japan2

Received 2 July 2001/ Accepted 17 October 2001

The Clostridium josui aga27A gene encodes the cellulosomal {alpha}-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various {alpha}-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A {alpha}-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.

* Corresponding author. Mailing address: Faculty of Bioresources, Mie University, 1515 Kamihamacho, Tsu 514-8507, Japan. Phone: (81) 59-231-9621. Fax: (81) 59-231-9684. E-mail: sakka{at}bio.mie-u.ac.jp.

Journal of Bacteriology, January 2002, p. 600-604, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.600-604.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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