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Journal of Bacteriology, January 2002, p. 600-604, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.600-604.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome

Faculty of Bioresources, Mie University, Tsu 514-8507, Japan,¹ Nagoya Seiraku Co. Ltd., Nagoya 468-8588, Japan²

Received 2 July 2001/ Accepted 17 October 2001

The Clostridium josui aga27A gene encodes the cellulosomal -galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various -galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A -galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.

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