This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fedhila, S.
Right arrow Articles by Lereclus, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fedhila, S.
Right arrow Articles by Lereclus, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5554-5562, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5554-5562.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Distinct clpP Genes Control Specific Adaptive Responses in Bacillus thuringiensis

Sinda Fedhila,1* Tarek Msadek,2 Patricia Nel,1 and Didier Lereclus1,2

Unité de Recherches de Lutte Biologique, Institut National de la Recherche Agronomique, 78285 Guyancourt Cedex,1 Unité de Biochimie Microbienne, Centre National de la Recherche Scientifique URA2172, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 6 May 2002/ Accepted 18 July 2002

ClpP and ClpC are subunits of the Clp ATP-dependent protease, which is ubiquitous among prokaryotic and eukaryotic organisms. The role of these proteins in stress tolerance, stationary-phase adaptive responses, and virulence in many bacterial species has been demonstrated. Based on the amino acid sequences of the Bacillus subtilis clpC and clpP genes, we identified one clpC gene and two clpP genes (designated clpP1 and clpP2) in Bacillus thuringiensis. Predicted proteins ClpP1 and ClpP2 have approximately 88 and 67% amino acid sequence identity with ClpP of B. subtilis, respectively. Inactivation of clpC in B. thuringiensis impaired sporulation efficiency. The clpP1 and clpP2 mutants were both slightly susceptible to salt stress, whereas disruption of clpP2 negatively affected sporulation and abolished motility. Virulence of the clp mutants was assessed by injecting bacteria into the hemocoel of Bombyx mori larvae. The clpP1 mutant displayed attenuated virulence, which appeared to be related to its inability to grow at low temperature (25°C), suggesting an essential role for ClpP1 in tolerance of low temperature. Microscopic examination of clpP1 mutant cells grown at 25°C showed altered bacterial division, with cells remaining attached after septum formation. Analysis of lacZ transcriptional fusions showed that clpP1 was expressed at 25 and 37°C during the entire growth cycle. In contrast, clpP2 was expressed at 37°C but not at 25°C, suggesting that ClpP2 cannot compensate for the absence of ClpP1 in the clpP1 mutant cells at low temperature. Our study demonstrates that ClpP1 and ClpP2 control distinct cellular regulatory pathways in B. thuringiensis.

* Corresponding author. Mailing address: Unité de Recherches de Lutte Biologique, Institut National de la Recherche Agronomique, La Minière, 78285 Guyancourt Cedex, France. Phone: 33 1 30 83 36 36. Fax: 33 1 30 43 80 97. E-mail: sindah{at}jouy.inra.fr.

Journal of Bacteriology, October 2002, p. 5554-5562, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5554-5562.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tseng, C.-L., Shaw, G.-C. (2008). Genetic Evidence for the Actin Homolog Gene mreBH and the Bacitracin Resistance Gene bcrC as Targets of the Alternative Sigma Factor SigI of Bacillus subtilis. J. Bacteriol. 190: 1561-1567 [Abstract] [Full Text]  
  • Lin, J.-S., Shaw, G.-C. (2007). Regulation of the kduID operon of Bacillus subtilis by the KdgR repressor and the ccpA gene: identification of two KdgR-binding sites within the kdgR-kduI intergenic region. Microbiology 153: 701-710 [Abstract] [Full Text]  
  • Tseng, C.-L., Chen, H.-J., Shaw, G.-C. (2006). Identification and Characterization of the Bacillus thuringiensis phaZ Gene, Encoding New Intracellular Poly-3-Hydroxybutyrate Depolymerase. J. Bacteriol. 188: 7592-7599 [Abstract] [Full Text]  
  • Crapoulet, N., Barbry, P., Raoult, D., Renesto, P. (2006). Global Transcriptome Analysis of Tropheryma whipplei in Response to Temperature Stresses.. J. Bacteriol. 188: 5228-5239 [Abstract] [Full Text]  
  • Ventura, M., Zhang, Z., Cronin, M., Canchaya, C., Kenny, J. G., Fitzgerald, G. F., van Sinderen, D. (2005). The ClgR Protein Regulates Transcription of the clpP Operon in Bifidobacterium breve UCC 2003. J. Bacteriol. 187: 8411-8426 [Abstract] [Full Text]  
  • Bouillaut, L., Ramarao, N., Buisson, C., Gilois, N., Gohar, M., Lereclus, D., Nielsen-LeRoux, C. (2005). FlhA Influences Bacillus thuringiensis PlcR-Regulated Gene Transcription, Protein Production, and Virulence. Appl. Environ. Microbiol. 71: 8903-8910 [Abstract] [Full Text]  
  • Fedhila, S., Guillemet, E., Nel, P., Lereclus, D. (2004). Characterization of Two Bacillus thuringiensis Genes Identified by In Vivo Screening of Virulence Factors. Appl. Environ. Microbiol. 70: 4784-4791 [Abstract] [Full Text]  
  • Bellier, A., Mazodier, P. (2004). ClgR, a Novel Regulator of clp and lon Expression in Streptomyces. J. Bacteriol. 186: 3238-3248 [Abstract] [Full Text]  
  • Beltramo, C., Grandvalet, C., Pierre, F., Guzzo, J. (2004). Evidence for Multiple Levels of Regulation of Oenococcus oeni clpP-clpL Locus Expression in Response to Stress. J. Bacteriol. 186: 2200-2205 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»