This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Darmon, E.
Right arrow Articles by Dijl, J. M. v.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Darmon, E.
Right arrow Articles by Dijl, J. M. v.
Right arrowPubmed/NCBI databases
*Substance via MeSH

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5661-5671, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5661-5671.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Novel Class of Heat and Secretion Stress-Responsive Genes Is Controlled by the Autoregulated CssRS Two-Component System of Bacillus subtilis

Elise Darmon,1 David Noone,2 Anne Masson,3 Sierd Bron,1 Oscar P. Kuipers,1 Kevin M. Devine,2* and Jan Maarten van Dijl4

Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, 9751 NN Haren,1 Department of Pharmaceutical Biology, University of Groningen, 9713 AV Groningen, The Netherlands,4 Department of Genetics, Smurfit Institute, Trinity College, Dublin 2, Ireland,2 Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France3

Received 7 March 2002/ Accepted 16 July 2002

Bacteria need dedicated systems that allow appropriate adaptation to the perpetual changes in their environments. In Bacillus subtilis, two HtrA-like proteases, HtrA and HtrB, play critical roles in the cellular response to secretion and heat stresses. Transcription of these genes is induced by the high-level production of a secreted protein or by a temperature upshift. The CssR-CssS two-component regulatory system plays an essential role in this transcriptional activation. Transcription of the cssRS operon is autoregulated and can be induced by secretion stress, by the absence of either HtrA or HtrB, and by heat stress in a HtrA null mutant strain. Two start sites are used for cssRS transcription, only one of which is responsive to heat and secretion stress. The divergently transcribed htrB and cssRS genes share a regulatory region through which their secretion and heat stress-induced expression is linked. This study shows that CssRS-regulated genes represent a novel class of heat-inducible genes, which is referred to as class V and currently includes two genes: htrA and htrB.

* Corresponding author: Mailing address: Department of Genetics, Smurfit Institute, Trinity College, Dublin 2, Ireland. Phone (353)-1-6081872. Fax: (353)-1-6798558. E-mail: kdevine{at}tcd.ie.

Journal of Bacteriology, October 2002, p. 5661-5671, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5661-5671.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sanchez, B., Champomier-Verges, M.-C., Stuer-Lauridsen, B., Ruas-Madiedo, P., Anglade, P., Baraige, F., de los Reyes-Gavilan, C. G., Johansen, E., Zagorec, M., Margolles, A. (2007). Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach. Appl. Environ. Microbiol. 73: 6757-6767 [Abstract] [Full Text]  
  • Lulko, A. T., Veening, J.-W., Buist, G., Smits, W. K., Blom, E. J., Beekman, A. C., Bron, S., Kuipers, O. P. (2007). Production and Secretion Stress Caused by Overexpression of Heterologous {alpha}-Amylase Leads to Inhibition of Sporulation and a Prolonged Motile Phase in Bacillus subtilis. Appl. Environ. Microbiol. 73: 5354-5362 [Abstract] [Full Text]  
  • Hyyrylainen, H.-L., Pietiainen, M., Lunden, T., Ekman, A., Gardemeister, M., Murtomaki-Repo, S., Antelmann, H., Hecker, M., Valmu, L., Sarvas, M., Kontinen, V. P. (2007). The density of negative charge in the cell wall influences two-component signal transduction in Bacillus subtilis. Microbiology 153: 2126-2136 [Abstract] [Full Text]  
  • Nijland, R., Heerlien, R., Hamoen, L. W., Kuipers, O. P. (2007). Changing a Single Amino Acid in Clostridium perfringens {beta}-Toxin Affects the Efficiency of Heterologous Secretion by Bacillus subtilis. Appl. Environ. Microbiol. 73: 1586-1593 [Abstract] [Full Text]  
  • Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., Czaplewski, L. (2007). Essential Bacterial Functions Encoded by Gene Pairs. J. Bacteriol. 189: 591-602 [Abstract] [Full Text]  
  • Darmon, E., Dorenbos, R., Meens, J., Freudl, R., Antelmann, H., Hecker, M., Kuipers, O. P., Bron, S., Quax, W. J., Dubois, J.-Y. F., van Dijl, J. M. (2006). A Disulfide Bond-Containing Alkaline Phosphatase Triggers a BdbC-Dependent Secretion Stress Response in Bacillus subtilis.. Appl. Environ. Microbiol. 72: 6876-6885 [Abstract] [Full Text]  
  • Chitlaru, T., Gat, O., Gozlan, Y., Ariel, N., Shafferman, A. (2006). Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities.. J. Bacteriol. 188: 3551-3571 [Abstract] [Full Text]  
  • Chhabra, S. R., He, Q., Huang, K. H., Gaucher, S. P., Alm, E. J., He, Z., Hadi, M. Z., Hazen, T. C., Wall, J. D., Zhou, J., Arkin, A. P., Singh, A. K. (2006). Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.. J. Bacteriol. 188: 1817-1828 [Abstract] [Full Text]  
  • Dalton, T. L., Collins, J. T., Barnett, T. C., Scott, J. R. (2006). RscA, a Member of the MDR1 Family of Transporters, Is Repressed by CovR and Required for Growth of Streptococcus pyogenes under Heat Stress. J. Bacteriol. 188: 77-85 [Abstract] [Full Text]  
  • Westers, H., Braun, P. G., Westers, L., Antelmann, H., Hecker, M., Jongbloed, J. D. H., Yoshikawa, H., Tanaka, T., van Dijl, J. M., Quax, W. J. (2005). Genes Involved in SkfA Killing Factor Production Protect a Bacillus subtilis Lipase against Proteolysis. Appl. Environ. Microbiol. 71: 1899-1908 [Abstract] [Full Text]  
  • Holtmann, G., Brigulla, M., Steil, L., Schutz, A., Barnekow, K., Volker, U., Bremer, E. (2004). RsbV-Independent Induction of the SigB-Dependent General Stress Regulon of Bacillus subtilis during Growth at High Temperature. J. Bacteriol. 186: 6150-6158 [Abstract] [Full Text]  
  • Kock, H., Gerth, U., Hecker, M. (2004). The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis. J. Bacteriol. 186: 5856-5864 [Abstract] [Full Text]  
  • Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
  • Westers, H., Dorenbos, R., van Dijl, J. M., Kabel, J., Flanagan, T., Devine, K. M., Jude, F., Seror, S. J., Beekman, A. C., Darmon, E., Eschevins, C., de Jong, A., Bron, S., Kuipers, O. P., Albertini, A. M., Antelmann, H., Hecker, M., Zamboni, N., Sauer, U., Bruand, C., Ehrlich, D. S., Alonso, J. C., Salas, M., Quax, W. J. (2003). Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis. Mol Biol Evol 20: 2076-2090 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»