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Journal of Bacteriology, October 2002, p. 5678-5685, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5678-5685.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biofilm Formation and Acyl Homoserine Lactone Production in the Burkholderia cepacia Complex

Barbara-Ann D. Conway, Vicnays Venu, and David P. Speert*

Department of Paediatrics, University of British Columbia, BC Research Institute for Children's and Women's Health, Vancouver, British Columbia, Canada V5Z 4H4

Received 24 April 2002/ Accepted 22 July 2002

Acyl homoserine lactone (acyl-HSL)-mediated gene regulation has been shown to influence biofilm formation in one Burkholderia cepacia cystic fibrosis isolate, but it is not known whether this relationship is a consistent feature of the several genomic species that make up the B. cepacia complex (BCC). We screened strains belonging to genomovars I to V of the BCC for biofilm formation on an abiotic surface and for acyl-HSL synthesis. We determined that organisms from each of these genomovars were capable of biofilm formation. Similarly, acyl-HSL was synthesized by organisms from each of genomovars I to V, with most isolates producing octanoyl-HSL in greatest abundance. When biofilms were grown in Luria broth, acyl-HSL synthesis and biofilm formation appeared to be associated, but these phenotypes were independent when the biofilms were grown in basal salts containing citrate. Genomovar V strains synthesized the greatest quantities of acyl-HSL, and genomovar II and III-A strains elaborated the most abundant biofilms. Quorum sensing may play a role in BCC pathogenesis, but it may not regulate biofilm formation under all growth conditions.

* Corresponding author. Mailing address: BC Research Institute for Children's and Women's Health, 950 West 28th Ave., Vancouver, BC, Canada V5Z 4H4. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}interchange.ubc.ca.

Journal of Bacteriology, October 2002, p. 5678-5685, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5678-5685.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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