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Journal of Bacteriology, October 2002, p. 5810-5813, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5810-5813.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Locus Required for Expression of High-Level Macrolide-Lincosamide-Streptogramin B Resistance in Staphylococcus aureus

P. K. Martin,^* Y. Bao, E. Boyer, K. M. Winterberg, L. McDowell, M. B. Schmid,^|| and J. M. Buysse

Essential Therapeutics, Inc., Mountain View, California 94043

Received 22 April 2002/ Accepted 15 July 2002

The yycF1(Ts) mutation in Staphylococcus aureus conferred hypersensitivity to macrolide-lincosamide-streptogramin B (MLS_B) antibiotics on strains either containing or lacking ermB. The overexpression of the S. aureus Ssa protein restored the yycF1 mutant to wild-type levels of susceptibility. Inactivation of ssa in an unmutagenized strain dramatically reduced ermB-based resistance. Conditional loss of function or expression of ssa in the yycF1 mutant is proposed to result in the observed hypersensitivity to MLS_B antibiotics.

Present address: Silicon Genetics, Inc., Redwood City, CA 94063.

Present address: University of Wisconsin, Department of Biochemistry, Madison, WI 53706-1544.

Present address: Antibacterial Molecular Sciences, Pfizer Global Research and Development, Ann Arbor, MI 48105.

^|| Present address: Affinium Pharmaceuticals, Inc., Toronto, Ontario M5J 1V6, Canada.

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