This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burr, S. E.
Right arrow Articles by Frey, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burr, S. E.
Right arrow Articles by Frey, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2002, p. 5966-5970, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.5966-5970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evidence for a Type III Secretion System in Aeromonas salmonicida subsp. salmonicida

Sarah E. Burr,1 Katja Stuber,1,{dagger} Thomas Wahli,2 and Joachim Frey1*

Institute of Veterinary Bacteriology,1 Centre for Fish and Wildlife Health, Institute of Animal Pathology, University of Berne, CH-3012 Berne, Switzerland2

Received 2 May 2002/ Accepted 5 August 2002

Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III secretion system. This has led to the identification of seven open reading frames that encode homologues to proteins functioning within the type III secretion systems of Yersinia species. Six of these proteins are encoded by genes comprising a virA operon. The A. salmonicida subsp. salmonicida yscV homologue, ascV, was inactivated by marker replacement mutagenesis and used to generate an isogenic ascV mutant. Comparison of the extracellular protein profiles from the ascV mutant and the wild-type strain indicates that A. salmonicida subsp. salmonicida secretes proteins via a type III secretion system. The recently identified ADP-ribosylating toxin AexT was identified as one such protein. Finally, we have compared the toxicities of the wild-type A. salmonicida subsp. salmonicida strain and the ascV mutant against RTG-2 rainbow trout gonad cells. While infection with the wild-type strain results in significant morphological changes, including cell rounding, infection with the ascV mutant has no toxic effect, indicating that the type III secretion system we have identified plays an important role in the virulence of this pathogen.

* Corresponding author. Mailing address: Institute of Veterinary Bacteriology, University of Berne, Laenggassstrasse 122 CH-3012, Berne, Switzerland. Phone: 41 31 6312 414. Fax: 41 31 6312 634. E-mail: joachim.frey{at}vbi.unibe.ch.

{dagger} Present address: Microbiological Laboratory, Swiss Federal Veterinary Office, Bern-Liebfeld, Switzerland.

Journal of Bacteriology, November 2002, p. 5966-5970, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.5966-5970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boyd, J. M., Dacanay, A., Knickle, L. C., Touhami, A., Brown, L. L., Jericho, M. H., Johnson, S. C., Reith, M. (2008). Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon (Salmo salar L.). Infect. Immun. 76: 1445-1455 [Abstract] [Full Text]  
  • Bossi-Kupfer, M., Genini, A., Peduzzi, R., Demarta, A. (2007). Tracheobronchitis caused by Aeromonas veronii biovar sobria after near-drowning. J Med Microbiol 56: 1563-1564 [Abstract] [Full Text]  
  • Fehr, D., Burr, S. E., Gibert, M., d'Alayer, J., Frey, J., Popoff, M. R. (2007). Aeromonas Exoenzyme T of Aeromonas salmonicida Is a Bifunctional Protein That Targets the Host Cytoskeleton. J. Biol. Chem. 282: 28843-28852 [Abstract] [Full Text]  
  • Fehr, D., Casanova, C., Liverman, A., Blazkova, H., Orth, K., Dobbelaere, D., Frey, J., Burr, S. E. (2006). AopP, a type III effector protein of Aeromonas salmonicida, inhibits the NF-{kappa}B signalling pathway.. Microbiology 152: 2809-2818 [Abstract] [Full Text]  
  • Dacanay, A., Knickle, L., Solanky, K. S., Boyd, J. M., Walter, J. A., Brown, L. L., Johnson, S. C., Reith, M. (2006). Contribution of the type III secretion system (TTSS) to virulence of Aeromonas salmonicida subsp. salmonicida. Microbiology 152: 1847-1856 [Abstract] [Full Text]  
  • Ebanks, R. O., Knickle, L. C., Goguen, M., Boyd, J. M., Pinto, D. M., Reith, M., Ross, N. W. (2006). Expression of and secretion through the Aeromonas salmonicida type III secretion system.. Microbiology 152: 1275-1286 [Abstract] [Full Text]  
  • Erova, T. E., Pillai, L., Fadl, A. A., Sha, J., Wang, S., Galindo, C. L., Chopra, A. K. (2006). DNA Adenine Methyltransferase Influences the Virulence of Aeromonas hydrophila. Infect. Immun. 74: 410-424 [Abstract] [Full Text]  
  • Broms, J. E., Edqvist, P. J., Carlsson, K. E., Forsberg, A., Francis, M. S. (2005). Mapping of a YscY Binding Domain within the LcrH Chaperone That Is Required for Regulation of Yersinia Type III Secretion. J. Bacteriol. 187: 7738-7752 [Abstract] [Full Text]  
  • Sha, J., Pillai, L., Fadl, A. A., Galindo, C. L., Erova, T. E., Chopra, A. K. (2005). The Type III Secretion System and Cytotoxic Enterotoxin Alter the Virulence of Aeromonas hydrophila. Infect. Immun. 73: 6446-6457 [Abstract] [Full Text]  
  • Burr, S. E., Pugovkin, D., Wahli, T., Segner, H., Frey, J. (2005). Attenuated virulence of an Aeromonas salmonicida subsp. salmonicida type III secretion mutant in a rainbow trout model. Microbiology 151: 2111-2118 [Abstract] [Full Text]  
  • Ghosh, P. (2004). Process of Protein Transport by the Type III Secretion System. Microbiol. Mol. Biol. Rev. 68: 771-795 [Abstract] [Full Text]  
  • Vilches, S., Urgell, C., Merino, S., Chacon, M. R., Soler, L., Castro-Escarpulli, G., Figueras, M. J., Tomas, J. M. (2004). Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain. Appl. Environ. Microbiol. 70: 6914-6919 [Abstract] [Full Text]  
  • Yu, H. B., Rao, P. S. S., Lee, H. C., Vilches, S., Merino, S., Tomas, J. M., Leung, K. Y. (2004). A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis. Infect. Immun. 72: 1248-1256 [Abstract] [Full Text]  
  • Chacon, M. R., Soler, L., Groisman, E. A., Guarro, J., Figueras, M. J. (2004). Type III Secretion System Genes in Clinical Aeromonas Isolates. J. Clin. Microbiol. 42: 1285-1287 [Abstract] [Full Text]  
  • Garrity-Ryan, L., Shafikhani, S., Balachandran, P., Nguyen, L., Oza, J., Jakobsen, T., Sargent, J., Fang, X., Cordwell, S., Matthay, M. A., Engel, J. N. (2004). The ADP Ribosyltransferase Domain of Pseudomonas aeruginosa ExoT Contributes to Its Biological Activities. Infect. Immun. 72: 546-558 [Abstract] [Full Text]  
  • Burr, S. E., Stuber, K., Frey, J. (2003). The ADP-Ribosylating Toxin, AexT, from Aeromonas salmonicida subsp. salmonicida Is Translocated via a Type III Secretion Pathway. J. Bacteriol. 185: 6583-6591 [Abstract] [Full Text]  
  • Stuber, K., Burr, S. E., Braun, M., Wahli, T., Frey, J. (2003). Type III Secretion Genes in Aeromonas salmonicida subsp. salmonicida Are Located on a Large Thermolabile Virulence Plasmid. J. Clin. Microbiol. 41: 3854-3856 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»