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Journal of Bacteriology, November 2002, p. 6065-6068, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6065-6068.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Gene yjfQ Encodes the Repressor of the yjfR-X Regulon (ula), Which Is Involved in L-Ascorbate Metabolism in Escherichia coli

Evangelina Campos, Juan Aguilar,* Laura Baldoma, and Josefa Badia

Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain

Received 14 June 2002/ Accepted 31 July 2002

Mutations in yjfQ allowed us to identify this gene as the regulator of the operon yjfS-X (ula operon), reported to be involved in L-ascorbate metabolism. Inactivation of this gene renders constitutive the expression of the ula operon, indicating that YjfQ acts as a repressor. We also demonstrate that this repressor regulates the nearby yjfR gene, which in this way constitutes a regulon with the ula operon.


* Corresponding author. Mailing address: Departamento de Bioquímica, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain. Phone: 34 93 403 4496. Fax: 34 93 402 4520. E-mail: jaguilar{at}farmacia.far.ub.es.


Journal of Bacteriology, November 2002, p. 6065-6068, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6065-6068.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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