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Journal of Bacteriology, November 2002, p. 6069-6072, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.183.21.6069-6072.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

groEL Expression in gyrB Mutants of Borrelia burgdorferi

Janet Alverson1,2* and D. Scott Samuels1

Division of Biological Sciences, The University of Montana, Missoula, Montana 59812,1 Biological Control and Mass Rearing Research Unit, USDA Agricultural Research Service, Mississippi State, Mississippi 397622

Received 27 March 2002/ Accepted 5 August 2002

GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.

* Corresponding author. Mailing address: USDA-ARS, Biological Control and Mass Rearing Research Unit, P.O. Box 5367, Mississippi State, MS 39762-5367. Phone: (662) 320-7443. Fax: (662) 320-7571. E-mail: janalverson{at}msa-msstate.ars.usda.gov.

Journal of Bacteriology, November 2002, p. 6069-6072, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.183.21.6069-6072.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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