This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allen, S. Articles by Downs, D. M. Search for Related Content PubMed PubMed Citation Articles by Allen, S. Articles by Downs, D. M.

Metabolic Flux in Both the Purine Mononucleotide and Histidine Biosynthetic Pathways Can Influence Synthesis of the Hydroxymethyl Pyrimidine Moiety of Thiamine in Salmonella enterica

Shara Allen, Julie L. Zilles, and Diana M. Downs^*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 6 August 2002/ Accepted 21 August 2002

Together, the biosyntheses of histidine, purines, and thiamine pyrophosphate (TPP) contain examples of convergent, divergent, and regulatory pathway integration. Mutations in two purine biosynthetic genes (purI and purH) affect TPP biosynthesis due to flux through the purine and histidine pathways. The molecular genetic characterization of purI mutants and their respective pseudorevertants resulted in the conclusion that <1% of the wild-type activity of the PurI enzyme was sufficient for thiamine but not for purine synthesis. The respective pseudorevertants were found to be informational suppressors. In addition, it was shown that accumulation of the purine intermediate aminoimidazole carboxamide ribotide inhibits thiamine synthesis, specifically affecting the conversion of aminoimidazole ribotide to hydroxymethyl pyrimidine.

Present address: Department of Civil and Environmental Engineering, University of Illinois—Urbana Champaign, Urbana, IL 61801.

This article has been cited by other articles:

• Ramos, I., Vivas, E. I., Downs, D. M. (2008). Mutations in the Tryptophan Operon Allow PurF-Independent Thiamine Synthesis by Altering Flux In Vivo. J. Bacteriol. 190: 815-822 [Abstract] [Full Text]  
• Dougherty, M. J., Boyd, J. M., Downs, D. M. (2006). Inhibition of Fructose-1,6-bisphosphatase by Aminoimidazole Carboxamide Ribotide Prevents Growth of Salmonella enterica purH Mutants on Glycerol. J. Biol. Chem. 281: 33892-33899 [Abstract] [Full Text]  
• Dougherty, M. J., Downs, D. M. (2006). A connection between iron-sulfur cluster metabolism and the biosynthesis of 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate in Salmonella enterica.. Microbiology 152: 2345-2353 [Abstract] [Full Text]  
• Dougherty, M. J., Downs, D. M. (2004). A Mutant Allele of rpoD Results in Increased Conversion of Aminoimidazole Ribotide to Hydroxymethyl Pyrimidine in Salmonella enterica. J. Bacteriol. 186: 4034-4037 [Abstract] [Full Text]  
• Ramos, I., Downs, D. M. (2003). Anthranilate Synthase Can Generate Sufficient Phosphoribosyl Amine for Thiamine Synthesis in Salmonella enterica. J. Bacteriol. 185: 5125-5132 [Abstract] [Full Text]  
• Dougherty, M., Downs, D. M. (2003). The stm4066 Gene Product of Salmonella enterica Serovar Typhimurium Has Aminoimidazole Riboside (AIRs) Kinase Activity and Allows AIRs To Satisfy the Thiamine Requirement of pur Mutant Strains. J. Bacteriol. 185: 332-339 [Abstract] [Full Text]