This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Masuda, N.
Right arrow Articles by Church, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masuda, N.
Right arrow Articles by Church, G. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2002, p. 6225-6234, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6225-6234.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Escherichia coli Gene Expression Responsive to Levels of the Response Regulator EvgA

Nobuhisa Masuda and George M. Church*

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 8 January 2002/ Accepted 22 August 2002

To investigate the function of the EvgA response regulator, we compared the genome-wide transcription profile of EvgA-overexpressing and EvgA-lacking Escherichia coli strains by oligonucleotide microarrays. The microarray measurements allowed the identification of at least 37 EvgA-activated genes, including acid resistance-related genes gadABC and hdeAB, efflux pump genes yhiUV and emrK, and 21 genes with unknown function. EvgA overexpression conferred acid resistance to exponentially growing cells. This acid resistance was abolished by deletion of ydeP, ydeO, or yhiE, which was induced by EvgA overexpression. These results suggest that ydeP, ydeO, and yhiE are novel genes related to acid resistance and that EvgA regulates several acid resistance genes. Furthermore, the deletion of yhiE completely abolished acid resistance in stationary-phase cells, suggesting that YhiE plays a critical role in stationary-phase acid resistance. The multidrug resistance in an acrB deletion mutant caused by EvgA overexpression was completely abolished by deletion of yhiUV, while the emrKY deletion had no effect on the increase in resistance by EvgA overexpression. In addition, EvgA overexpression did not confer resistance in a tolC-deficient strain. These results suggest that YhiUV induced by EvgA overexpression is functionally associated with TolC and contributes to multidrug resistance.

* Corresponding author. Mailing address: Department of Genetics, Warren Alpert Building, Room 513, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-7561. Fax: (617) 432-7266. E-mail: church{at}arep.med.harvard.edu.

Journal of Bacteriology, November 2002, p. 6225-6234, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6225-6234.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ghosh, D., Roy, K., Williamson, K. E., Srinivasiah, S., Wommack, K. E., Radosevich, M. (2009). Acyl-Homoserine Lactones Can Induce Virus Production in Lysogenic Bacteria: an Alternative Paradigm for Prophage Induction. Appl. Environ. Microbiol. 75: 7142-7152 [Abstract] [Full Text]  
  • Toyota, C. G., Berthold, C. L., Gruez, A., Jonsson, S., Lindqvist, Y., Cambillau, C., Richards, N. G. J. (2008). Differential Substrate Specificity and Kinetic Behavior of Escherichia coli YfdW and Oxalobacter formigenes Formyl Coenzyme A Transferase. J. Bacteriol. 190: 2556-2564 [Abstract] [Full Text]  
  • Christ, D., Chin, J. W. (2008). Engineering Escherichia coli heat-resistance by synthetic gene amplification. Protein Eng Des Sel 21: 121-125 [Abstract] [Full Text]  
  • DeVito, J. A. (2008). Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli. Nucleic Acids Res 36: e4-e4 [Abstract] [Full Text]  
  • Eguchi, Y., Itou, J., Yamane, M., Demizu, R., Yamato, F., Okada, A., Mori, H., Kato, A., Utsumi, R. (2007). B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli. Proc. Natl. Acad. Sci. USA 104: 18712-18717 [Abstract] [Full Text]  
  • Richard, H., Foster, J. W. (2007). Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE. Microbiology 153: 3154-3161 [Abstract] [Full Text]  
  • Sayed, A. K., Odom, C., Foster, J. W. (2007). The Escherichia coli AraC-family regulators GadX and GadW activate gadE, the central activator of glutamate-dependent acid resistance. Microbiology 153: 2584-2592 [Abstract] [Full Text]  
  • Bohnert, J. A., Schuster, S., Fahnrich, E., Trittler, R., Kern, W. V. (2007). Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF). J Antimicrob Chemother 59: 1216-1222 [Abstract] [Full Text]  
  • Mates, A. K., Sayed, A. K., Foster, J. W. (2007). Products of the Escherichia coli Acid Fitness Island Attenuate Metabolite Stress at Extremely Low pH and Mediate a Cell Density-Dependent Acid Resistance. J. Bacteriol. 189: 2759-2768 [Abstract] [Full Text]  
  • Castanie-Cornet, M.-P., Treffandier, H., Francez-Charlot, A., Gutierrez, C., Cam, K. (2007). The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His-Asp phosphorelay RcsCDB/AF. Microbiology 153: 238-246 [Abstract] [Full Text]  
  • Brodie, E. L., DeSantis, T. Z., Joyner, D. C., Baek, S. M., Larsen, J. T., Andersen, G. L., Hazen, T. C., Richardson, P. M., Herman, D. J., Tokunaga, T. K., Wan, J. M., Firestone, M. K. (2006). Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation. Appl. Environ. Microbiol. 72: 6288-6298 [Abstract] [Full Text]  
  • Lin, C.-T., Huang, T.-Y., Liang, W.-C., Peng, H.-L. (2006). Homologous Response Regulators KvgA, KvhA and KvhR Regulate the Synthesis of Capsular Polysaccharide in Klebsiella pneumoniae CG43 in a Coordinated Manner. J Biochem 140: 429-438 [Abstract] [Full Text]  
  • Schlicht, M., Berens, C., Daam, J., Hillen, W. (2006). Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression. Appl. Environ. Microbiol. 72: 5637-5642 [Abstract] [Full Text]  
  • Gurvich, O. L., Baranov, P. V., Gesteland, R. F., Atkins, J. F. (2005). Expression Levels Influence Ribosomal Frameshifting at the Tandem Rare Arginine Codons AGG_AGG and AGA_AGA in Escherichia coli. J. Bacteriol. 187: 4023-4032 [Abstract] [Full Text]  
  • van Diemen, P. M., Dziva, F., Stevens, M. P., Wallis, T. S. (2005). Identification of Enterohemorrhagic Escherichia coli O26:H- Genes Required for Intestinal Colonization in Calves. Infect. Immun. 73: 1735-1743 [Abstract] [Full Text]  
  • Zwir, I., Shin, D., Kato, A., Nishino, K., Latifi, T., Solomon, F., Hare, J. M., Huang, H., Groisman, E. A. (2005). Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proc. Natl. Acad. Sci. USA 102: 2862-2867 [Abstract] [Full Text]  
  • Lee, L. J., Barrett, J. A., Poole, R. K. (2005). Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc. J. Bacteriol. 187: 1124-1134 [Abstract] [Full Text]  
  • Lucchini, S., Liu, H., Jin, Q., Hinton, J. C. D., Yu, J. (2005). Transcriptional Adaptation of Shigella flexneri during Infection of Macrophages and Epithelial Cells: Insights into the Strategies of a Cytosolic Bacterial Pathogen. Infect. Immun. 73: 88-102 [Abstract] [Full Text]  
  • Ma, Z., Masuda, N., Foster, J. W. (2004). Characterization of EvgAS-YdeO-GadE Branched Regulatory Circuit Governing Glutamate-Dependent Acid Resistance in Escherichia coli. J. Bacteriol. 186: 7378-7389 [Abstract] [Full Text]  
  • Eguchi, Y., Okada, T., Minagawa, S., Oshima, T., Mori, H., Yamamoto, K., Ishihama, A., Utsumi, R. (2004). Signal Transduction Cascade between EvgA/EvgS and PhoP/PhoQ Two-Component Systems of Escherichia coli. J. Bacteriol. 186: 3006-3014 [Abstract] [Full Text]  
  • Arends, S. J. R., Weiss, D. S. (2004). Inhibiting Cell Division in Escherichia coli Has Little If Any Effect on Gene Expression. J. Bacteriol. 186: 880-884 [Abstract] [Full Text]  
  • Hommais, F., Krin, E., Coppee, J.-Y., Lacroix, C., Yeramian, E., Danchin, A., Bertin, P. (2004). GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli. Microbiology 150: 61-72 [Abstract] [Full Text]  
  • Eguchi, Y., Oshima, T., Mori, H., Aono, R., Yamamoto, K., Ishihama, A., Utsumi, R. (2003). Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli. Microbiology 149: 2819-2828 [Abstract] [Full Text]  
  • Tucker, D. L., Tucker, N., Ma, Z., Foster, J. W., Miranda, R. L., Cohen, P. S., Conway, T. (2003). Genes of the GadX-GadW Regulon in Escherichia coli. J. Bacteriol. 185: 3190-3201 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»