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Journal of Bacteriology, November 2002, p. 6235-6249, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6235-6249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phenotypes of lexA Mutations in Salmonella enterica: Evidence for a Lethal lexA Null Phenotype Due to the Fels-2 Prophage

Kim Bunny,{dagger} Jing Liu,{ddagger} and John Roth{dagger}*

Department of Biology, University of Utah, Salt Lake City, Utah 84112

Received 19 February 2002/ Accepted 18 August 2002

The LexA protein of Escherichia coli represses the damage-inducible SOS regulon, which includes genes for repair of DNA. Surprisingly, lexA null mutations in Salmonella enterica are lethal even with a sulA mutation, which corrects lexA lethality in E. coli. Nine suppressors of lethality isolated in a sulA mutant of S. enterica had lost the Fels-2 prophage, and seven of these (which grew better) had also lost the Gifsy-1 and Gifsy-2 prophages. All three phage genomes included a homologue of the tum gene of coliphage 186, which encodes a LexA-repressed cI antirepressor. The tum homologue of Fels-2 was responsible for lexA lethality and had a LexA-repressed promoter. This basis of lexA lethality was unexpected because the four prophages of S. enterica LT2 are not strongly UV inducible and do not sensitize strains to UV killing. In S. enterica, lexA(Ind-) mutants have the same phenotypes as their E. coli counterparts. Although lexA null mutants express their error-prone DinB polymerase constitutively, they are not mutators in either S. enterica or E. coli.

* Corresponding author. Present address: Microbiology Section of DBS, Center for Genetics and Development, University of California, Davis, Davis, CA 95616-8665. Phone: (530) 752-6679. Fax: (530) 752-7663. E-mail: jrroth{at}ucdavis.edu.

{dagger} Present address: Center for Genetics and Development, University of California, Davis, Davis, CA 95616-8665.

{ddagger} Present address: Stowers Institute for Medical Research, Kansas City, MO 64110.

Journal of Bacteriology, November 2002, p. 6235-6249, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6235-6249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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