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Journal of Bacteriology, November 2002, p. 6384-6386, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6384-6386.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Structural Heterogeneity of the Streptococcal C5a Peptidase Gene in Streptococcus pyogenes

Irina V. Koroleva,1 Androulla Efstratiou,2 and Alexander N. Suvorov1*

Institute of Experimental Medicine, St. Petersburg, Russia,1 PHLS Respiratory and Systemic Infection Laboratory, London, United Kingdom2

Received 14 June 2002/ Accepted 22 July 2002

The 3' ends of the genes for the C-terminal region of C5a peptidase from 15 Streptococcus pyogenes isolates were analyzed by PCR. Amplicons were found to differ in size. DNA sequence analysis revealed that the differences between PCR fragment sizes accorded with the number of R repeats in the C5a peptidase gene.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Institute of Experimental Medicine, acad. Pavlov's St. 12, 197376 St. Petersburg, Russia. E-mail: lab{at}suvorov.spb.su.

Journal of Bacteriology, November 2002, p. 6384-6386, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6384-6386.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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