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Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein

New England Biolabs, Beverly, Massachusetts 01915

Received 22 May 2002/ Accepted 16 August 2002

Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues.

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