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Journal of Bacteriology, December 2002, p. 6472-6480, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6472-6480.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa

Larry A. Gallagher,1 Susan L. McKnight,2 Marina S. Kuznetsova,1 Everett C. Pesci,2 and Colin Manoil1*

Department of Genome Sciences, University of Washington, Seattle, Washington 98195,1 Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina 278582

Received 22 May 2002/ Accepted 28 August 2002

A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in the primary phenazine biosynthetic pathway and those with more pleiotropic defects. The largest set of pleiotropic mutations blocked the production of the extracellular Pseudomonas quinolone signal (PQS), a molecule required for the synthesis of secondary metabolites and extracellular enzymes. Most of these pqs mutations affected genes which appear to encode PQS biosynthetic functions, although a transcriptional regulator and an apparent response effector were also represented. Two of the genes required for PQS synthesis (phnA and phnB) had previously been assumed to encode phenazine biosynthetic functions. The transcription of one of the genes required for PQS synthesis (PA2587/pqsH) was regulated by the LasI/R quorum-sensing system, thereby linking quorum sensing and PQS regulation. Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice.

* Corresponding author. Mailing address: Department of Genome Sciences, Box 357730, University of Washington, Seattle, WA 98195. Phone: (206) 543-7800. Fax: (206) 543-0754. E-mail: manoil{at}u.washington.edu.

Journal of Bacteriology, December 2002, p. 6472-6480, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6472-6480.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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