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Journal of Bacteriology, December 2002, p. 6592-6601, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6592-6601.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Vibrio cholerae Phage K139: Complete Genome Sequence and Comparative Genomics of Related Phages

Dagmar Kapfhammer,1 Julia Blass,1 Stefan Evers,2 and Joachim Reidl1*

Zentrum für Infektionsforschung, Universität Würzburg, 97070 Würzburg, Germany,1 Hoffmann LaRoche, CH-4058 Basel, Switzerland2

Received 5 July 2002/ Accepted 7 September 2002

In this report, we characterize the complete genome sequence of the temperate phage K139, which morphologically belongs to the Myoviridae phage family (P2 and 186). The prophage genome consists of 33,106 bp, and the overall GC content is 48.9%. Forty-four open reading frames were identified. Homology analysis and motif search were used to assign possible functions for the genes, revealing a close relationship to P2-like phages. By Southern blot screening of a Vibrio cholerae strain collection, two highly K139-related phage sequences were detected in non-O1, non-O139 strains. Combinatorial PCR analysis revealed almost identical genome organizations. One region of variable gene content was identified and sequenced. Additionally, the tail fiber genes were analyzed, leading to the identification of putative host-specific sequence variations. Furthermore, a K139-encoded Dam methyltransferase was characterized.

* Corresponding author. Mailing address: Zentrum für Infektionsforschung, Universität Würzburg, Röntgenring 11, 97070 Würzburg, Germany. Phone: (49) (0) 931 312153. Fax: (49) (0) 931 312578. E-mail: joachim.reidl{at}mail.uni-wuerzburg.de.

Journal of Bacteriology, December 2002, p. 6592-6601, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6592-6601.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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