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Journal of Bacteriology, December 2002, p. 6709-6713, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6709-6713.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Isolation of Glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a

Christina Schäffer,1* Anke I. Beckedorf,2,3 Andrea Scheberl,1 Sonja Zayni,1 Jasna Peter-Katalinic,2 and Paul Messner1

Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, A-1180 Vienna, Austria,1 Institut für Medizinische Physik und Biophysik, Universität Münster, D-48149 Münster, Germany,2 Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912-26203

Received 2 July 2002/ Accepted 3 September 2002

Glucose-substituted cardiolipins account for about 4 mol% of total phospholipid extracted from exponentially grown cells of Geobacillus stearothermophilus NRS 2004/3a. Individual glucocardiolipin species exhibited differences in fatty acid substitution, with iso-C15:0 and anteiso-C17:0 prevailing. The compounds were purified to homogeneity by a novel protocol and precharacterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

* Corresponding author. Mailing address: Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, Gregor-Mendel-Strasse 33, A-1180 Vienna, Austria. Phone: 0043-1-4789112, ext. 2203. Fax: 0043-1-4789112. E-mail: CRS{at}edv1.boku.ac.at.

Journal of Bacteriology, December 2002, p. 6709-6713, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6709-6713.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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