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Journal of Bacteriology, December 2002, p. 6714-6720, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6714-6720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Transcriptional Response of Pasteurella multocida to Defined Iron Sources

Michael L. Paustian,1 Barbara J. May,1 Dongwei Cao,2 Daniel Boley,2 and Vivek Kapur1*

Biomedical Genomics Center and Departments of Microbiology and Veterinary Pathobiology,1 Department of Computer Science, University of Minnesota, St. Paul, Minnesota 551082

Received 22 January 2002/ Accepted 4 September 2002

Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. This resulted in a set of data containing over 338,000 gene expression observations. On average, 12% of P. multocida genes were differentially expressed under any single condition. A majority of these genes encoded P. multocida proteins that were involved in either transport and binding or were annotated as hypothetical proteins. Several trends are evident when the data from different iron sources are compared. In general, only two genes (ptsN and sapD) were expressed at elevated levels under all of the conditions tested. The results also show that genes with increased expression in the presence of hemoglobin did not respond to transferrin or ferritin as an iron source. Correspondingly, genes with increased expression in the transferrin and ferritin experiments were expressed at reduced levels when hemoglobin was supplied as the sole iron source. Finally, the data show that genes that were most responsive to the presence of ferric citrate did not follow a trend similar to that of the other iron sources, suggesting that different pathways respond to inorganic or organic sources of iron in P. multocida. Taken together, our results demonstrate that unique subsets of P. multocida genes are expressed in response to different iron sources and that many of these genes have yet to be functionally characterized.

* Corresponding author. Mailing address: Biomedical Genomics Center and Department of Veterinary Pathobiology, University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN 55108. Phone: (612) 625-7712. Fax: (612) 625-5203. E-mail: vkapur{at}umn.edu.

Journal of Bacteriology, December 2002, p. 6714-6720, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6714-6720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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