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Journal of Bacteriology, December 2002, p. 6730-6733, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6730-6733.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli

Sean M. O'Donnell{dagger} and Gary R. Janssen*

Department of Microbiology, Miami University, Oxford, Ohio 45056

Received 15 March 2002/ Accepted 3 September 2002

By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.

* Corresponding author. Mailing address: Department of Microbiology, Miami University, Oxford, OH 45056. Phone: (513) 529-1694. Fax: (513) 529-2431. E-mail: janssegr{at}muohio.edu.

{dagger} Present address: Department of Pediatric Infectious Disease, Vanderbilt University School of Medicine, Nashville, TN 37232.

Journal of Bacteriology, December 2002, p. 6730-6733, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6730-6733.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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