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Journal of Bacteriology, December 2002, p. 6845-6858, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.6845-6858.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Global Gene Expression Profiles of the Cyanobacterium Synechocystis sp. Strain PCC 6803 in Response to Irradiation with UV-B and White Light

Lixuan Huang,* Michael P. McCluskey, Hao Ni, and Robert A. LaRossa

Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173

Received 17 June 2002/ Accepted 13 September 2002

We developed a transcript profiling methodology to elucidate expression patterns of the cyanobacterium Synechocystis sp. strain PCC 6803 and used the technology to investigate changes in gene expression caused by irradiation with either intermediate-wavelength UV light (UV-B) or high-intensity white light. Several families of transcripts were altered by UV-B treatment, including mRNAs specifying proteins involved in light harvesting, photosynthesis, photoprotection, and the heat shock response. In addition, UV-B light induced the stringent response in Synechocystis, as indicated by the repression of ribosomal protein transcripts and other mRNAs involved in translation. High-intensity white light- and UV-B-mediated expression profiles overlapped in the down-regulation of photosynthesis genes and induction of heat shock response but differed in several other transcriptional processes including those specifying carbon dioxide uptake and fixation, the stringent response, and the induction profile of the high-light-inducible proteins. These two profile comparisons not only corroborated known physiological changes but also suggested coordinated regulation of many pathways, including synchronized induction of D1 protein recycling and a coupling between decreased phycobilisome biosynthesis and increased phycobilisome degradation. Overall, the gene expression profile analysis generated new insights into the integrated network of genes that adapts rapidly to different wavelengths and intensities of light.

* Corresponding author. Mailing address: Central Research and Development, Biochemical Sciences and Engineering, Experimental Station, P.O. Box 80173, DuPont Company, Wilmington, DE 19880-0173. Phone: (302) 695-3471. Fax: (302) 695-9183. E-mail: Lisa.L.Huang{at}usa.dupont.com.

Journal of Bacteriology, December 2002, p. 6845-6858, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.6845-6858.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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