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Journal of Bacteriology, December 2002, p. 7042-7046, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7042-7046.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Analysis of Infection Thread Development Using Gfp- and DsRed-Expressing Sinorhizobium meliloti

Daniel J. Gage*

Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269

Received 2 May 2002/ Accepted 28 August 2002

Sinorhizobium meliloti growth inside infection threads was monitored after inoculation of alfalfa with red- or green-tagged bacteria. Most threads were populated with single bacterial types. Mixed infections were present but gave mixed nodules less often than expected. These patterns are explained by a model describing bacterial growth during infection.


* Mailing address: University of Connecticut, Department of Molecular and Cell Biology, 75 N. Eagleville Rd., U-44, Storrs, CT 06269. Phone: (860) 486-3092. Fax: (860) 486-1784. E-mail: gage{at}uconnvm.uconn.edu.


Journal of Bacteriology, December 2002, p. 7042-7046, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7042-7046.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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