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Journal of Bacteriology, December 2002, p. 7047-7054, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7047-7054.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The DnaK Chaperone Is Necessary for {alpha}-Complementation of ß-Galactosidase in Escherichia coli

Nicolas Lopes Ferreira and Jean-Hervé Alix*

Institut de Biologie Physico-Chimique, U.P.R. 9073 du C.N.R.S. and Université Paris 7-Denis Diderot, F-75005 Paris, France

Received 24 July 2002/ Returned for modification 29 August 2002/ Accepted 19 September 2002

We show here the involvement of the molecular chaperone DnaK from Escherichia coli in the in vivo {alpha}-complementation of the ß-galactosidase. In the dnaK756(Ts) mutant, {alpha}-complementation occurs when the organisms are grown at 30°C but not at 37 or 40°C, although these temperatures are permissive for bacterial growth. Plasmid-driven expression of wild-type dnaK restores the {alpha}-complementation in the mutant but also stimulates it in a dnaK+ strain. In a mutant which contains a disrupted dnaK gene ({Delta}dnaK52::Cmr), {alpha}-complementation is also impaired, even at 30°C. This observation provides an easy and original phenotype to detect subtle functional changes in a protein such as the DnaK756 chaperone, within the physiologically relevant temperature.

* Corresponding author. Mailing address: Institut de Biologie Physico-Chimique, U.P.R. 9073 du C.N.R.S., 13 rue Pierre et Marie Curie, F-75005 Paris, France. Phone: (33) (1) 58 41 51 35. Fax: (33) (1) 58 41 50 20. E-mail: alix{at}ibpc.fr.

Journal of Bacteriology, December 2002, p. 7047-7054, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7047-7054.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ferreira, N. L., Labbe, D., Monot, F., Fayolle-Guichard, F., Greer, C. W. (2006). Genes involved in the methyl tert-butyl ether (MTBE) metabolic pathway of Mycobacterium austroafricanum IFP 2012.. Microbiology 152: 1361-1374 [Abstract] [Full Text]  
  • ALIX, J.-H., NIERHAUS, K. H. (2003). DnaK-facilitated ribosome assembly in Escherichia coli revisited. RNA 9: 787-793 [Abstract] [Full Text]  


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