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 Previous Article

Journal of Bacteriology, December 2002, p. 7062-7067, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7062-7067.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida

María-Isabel Ramos-González,1* Monica Olson,2 Anthony A. Gatenby,2 Gilberto Mosqueda,3 Maximino Manzanera,1,{dagger} María J. Campos,1 Susana Víchez,1,{dagger} and Juan L. Ramos1

Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain,1 DuPont Experimental Station, Central Research and Development, Wilmington, Delaware 19880-0328,2 Department of Plant Genetics Engineering, Plant Biotechnology Unit, Centro de Investigación y Estudios Avanzados, 36500 Irapuato, Guanajuato, Mexico3

Received 11 July 2002/ Accepted 27 September 2002

The tmoABCDEF genes encode the toluene-4-monooxygenase from Pseudomonas mendocina KR1. Upstream from the tmoA gene an open reading frame, tmoX, encoding a protein 83% identical to TodX (todX being the initial gene in the todXFC1C2BADEGIH operon from Pseudomonas putida DOT-T1E) was found. The tmoX gene is also the initial gene in the tmoXABCDEF gene cluster. The transcription initiation point from the tmoX promoter was mapped, and the sequence upstream revealed striking identity with the promoter of the tod operon of P. putida. The tod operon is regulated by a two-component signal transduction system encoded by the todST genes. Two novel genes from P. mendocina KR1, tmoST, were rescued by complementation of a P. putida DOT-T1E todST knockout mutant, whose gene products shared about 85% identity with TodS-TodT. We show that transcription from PtmoX and PtodX can be mediated by TmoS-TmoT or TodS-TodT, in the presence of toluene, revealing cross-regulation between these two catabolic pathways.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Profesor Albareda, 1, 18008 Granada, Spain. Phone: 34-958-181600. Fax: 34-958-129600. E-mail: maribel.ramos{at}eez.csic.es.

{dagger} Present address: Institute of Biotechnology and Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom.

Journal of Bacteriology, December 2002, p. 7062-7067, Vol. 184, No. 24
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.24.7062-7067.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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