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Journal of Bacteriology, February 2002, p. 615-620, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.615-620.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Medium-Chain Prenyl Diphosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus solfataricus

Hisashi Hemmi, Satoru Ikejiri, Satoshi Yamashita, and Tokuzo Nishino*

Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

Received 18 June 2001/ Accepted 31 October 2001

Two open reading frames which encode the homologues of (all-E) prenyl diphosphate synthase are found in the whole-genome sequence of Sulfolobus solfataricus, a thermoacidophilic archaeon. It has been suggested that one is a geranylgeranyl diphosphate synthase gene, but the specificity and biological significance of the enzyme encoded by the other have remained unclear. Thus, we isolated the latter by the PCR method, expressed the enzyme in Escherichia coli cells, purified it, and characterized it. The archaeal enzyme, 281 amino acids long, is highly thermostable and requires Mg2+ and Triton X-100 for full activity. It catalyzes consecutive E-type condensations of isopentenyl diphosphate with an allylic substrate such as geranylgeranyl diphosphate and yields the medium-chain product hexaprenyl diphosphate. Despite such product specificity, phylogenetic analysis revealed that the archaeal medium-chain prenyl diphosphate synthase is distantly related to the other medium- and long-chain enzymes but is closely related to eucaryal short-chain enzymes.

* Corresponding author. Mailing address: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai, Miyagi 980-8579, Japan. Phone and fax: 81-22-217-7270. E-mail: nishino{at}mail.cc.tohoku.ac.jp.

Journal of Bacteriology, February 2002, p. 615-620, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.615-620.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sato, S., Murakami, M., Yoshimura, T., Hemmi, H. (2008). Specific Partial Reduction of Geranylgeranyl Diphosphate by an Enzyme from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Yields a Reactive Prenyl Donor, Not a Dead-End Product. J. Bacteriol. 190: 3923-3929 [Abstract] [Full Text]  
  • Sun, H.-Y., Ko, T.-P., Kuo, C.-J., Guo, R.-T., Chou, C.-C., Liang, P.-H., Wang, A. H.-J. (2005). Homodimeric Hexaprenyl Pyrophosphate Synthase from the Thermoacidophilic Crenarchaeon Sulfolobus solfataricus Displays Asymmetric Subunit Structures. J. Bacteriol. 187: 8137-8148 [Abstract] [Full Text]  


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