This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dailey, F. E. Articles by Macnab, R. M. Search for Related Content PubMed PubMed Citation Articles by Dailey, F. E. Articles by Macnab, R. M.

Effects of Lipoprotein Biogenesis Mutations on Flagellar Assembly in Salmonella

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114,¹ Department of Biology, College of Wooster, Wooster, Ohio 44691²

Received 8 August 2001/ Accepted 2 November 2001

Flagellar assembly requires the expression of a large number of flagellum-specific genes. However, mutations in a number of other genes in Salmonella and Escherichia coli have been shown to have pleiotropic effects that affect flagellar assembly. FlgH (the L-ring subunit of the flagellar basal body) is a lipoprotein whose modification is important for L-ring assembly. We therefore tested whether the lack of motility of Salmonella mutants defective in lipoprotein biogenesis is a result of inability to modify FlgH. Our results show that temperature-sensitive apolipoprotein N-acyltransferase [lnt(Ts)] mutants are nonflagellate at 42°C. However, the flagellar assembly defect occurs at a much earlier step in the pathway than L-ring assembly. These mutants failed to assemble even an MS ring, presumably because of the observed decrease in transcription of fliF. In contrast, temperature-sensitive diacylglycerol transferase [lgt(Ts)] mutants were motile at 42°C, provided the strains carried an lpp (Braun lipoprotein) mutation to permit growth. We have isolated second-site mutants from an lgt(Ts) lpp⁺ strain that grow but are nonflagellate at 42°C. Thus, lipoprotein biogenesis is a factor that is important for flagellar assembly.

This article has been cited by other articles:

• Lewenza, S., Vidal-Ingigliardi, D., Pugsley, A. P. (2006). Direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family enterobacteriaceae.. J. Bacteriol. 188: 3516-3524 [Abstract] [Full Text]  
• Yerushalmi, G., Zusman, T., Segal, G. (2005). Additive Effect on Intracellular Growth by Legionella pneumophila Icm/Dot Proteins Containing a Lipobox Motif. Infect. Immun. 73: 7578-7587 [Abstract] [Full Text]  
• Fadl, A. A., Sha, J., Klimpel, G. R., Olano, J. P., Niesel, D. W., Chopra, A. K. (2005). Murein Lipoprotein Is a Critical Outer Membrane Component Involved in Salmonella enterica Serovar Typhimurium Systemic Infection. Infect. Immun. 73: 1081-1096 [Abstract] [Full Text]  
• Sha, J., Fadl, A. A., Klimpel, G. R., Niesel, D. W., Popov, V. L., Chopra, A. K. (2004). The Two Murein Lipoproteins of Salmonella enterica Serovar Typhimurium Contribute to the Virulence of the Organism. Infect. Immun. 72: 3987-4003 [Abstract] [Full Text]  
• Kelly, A., Goldberg, M. D., Carroll, R. K., Danino, V., Hinton, J. C. D., Dorman, C. J. (2004). A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium. Microbiology 150: 2037-2053 [Abstract] [Full Text]